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Glucose oxidase ofAspergillus niger

II. Production of glucose oxidase by mixed cultures of different strains ofAspergillus niger

Глюкозооксидаза Aspergillus niger

II. Продукция глюкозооксидазы при смешанной культивации Aspergillus niger

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Abstract

The authors studied the possibility of utilizing the method of mixed cultivation of pairs of strains ofAspergillus niger for the production of glucose oxidase by submerged fermentation. Two methods of mixed cultivation were used: (1) inoculation of the fermentation medium with a mixture of the spores of two separately cultivated strains, (2) preparation of the spore inoculum by mixed surface cultivation of pairs of strains. In both cases some pairs of strains gave increased production of the enzyme, which was most marked in fermentation in semisynthetic medium. Mixed surface cultivation of strains 77 and A20 gave rise to spores which, when used as inoculum, led to a 37% increase in production of the enzyme in submerged cultivation in natural medium.

Abstract

Изучалась возможность использования метода смешанного культивирования двух штаммов Aspergillus niger для продукции глюкозооксидазы при глубинной ферментации. Применялись два метода смешанного культивирования: 1) посев в ферментационную среду смеєи спор двух штаммов, выращенных отдельно; 2) заготовка спор для посева путем поверхностной культивации смеси двух штаммов. В обоих случаях мы в некоторых комбинациях пар штаммов наблюдали повышение продукции ферментов, особенно заметное при ферментации в полусинтетической среде. Путем смешанной поверхностной культивации штаммов 77 и А20 были получены споры, при посеве которых продукцзя фермента при глубинной культивации в естественной среде повысилась на 37%.

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Pásková, J., Munk, V. Glucose oxidase ofAspergillus niger . Folia Microbiol 8, 215–220 (1963). https://doi.org/10.1007/BF02872584

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