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Artificial gravity loading increases the effects of the glutamate transporter inhibitors on the glutamate release and uptake in rat brain nerve terminals

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Abstract

The present study evaluated the effect of artificial gravity loading on transporter-mediated uptake and release of L-glutamate using the inhibitors of glutamate transporters as tools. The competitive nontransportable, DL-threo-beta-benzyloxyaspartate (DL-TBOA), and transportable, DL-threo-beta-hydroxyaspartate (DL-THA), inhibitors were demonstrated to better inhibit the L-[14C]glutamate uptake under centrifuge-induced hypergravity compared with the normal gravity control. The effect of DL-TBOA on depolarization-induced carrier-mediated L-[14C]glutamate release also increased after hypergravity loading in Na+- and low [Na+] NMDG- supplemented media. 10 µM DL-TBOA-induced decrease in L-[14C]glutamate release in Na+ — supplemented medium was 15.2±2.2 % in the control experiments and 26.2±3.9 % after centrifuge-induced loading (P≤0,05) and in low [Na+] medium was 37.0±2.5 % and 45.0±3.4 %, respectively.

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Authors and Affiliations

  1. Department of Neurochemistry, Palladin Institute of Biochemistry KAS of Ukraine, Kiev

    Tatiana Borisova, Natalia Krisanova & Nina Himmelreich

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  1. Tatiana Borisova
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  2. Natalia Krisanova
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  3. Nina Himmelreich
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Correspondence to Tatiana Borisova.

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Borisova, T., Krisanova, N. & Himmelreich, N. Artificial gravity loading increases the effects of the glutamate transporter inhibitors on the glutamate release and uptake in rat brain nerve terminals. Microgravity Sci. Technol 18, 230–233 (2006). https://doi.org/10.1007/BF02870416

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