Skip to main content
SpringerLink
Log in

Densitometry of yeast cells and protoplasts during sugar uptake

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Densitometry was applied to the study of sugar transport in yeast cells and protoplasts. The uptake curves of nonmetabolized monosaccharides were without the transient peak observed analytically. Differences in the space of distribution of different monosaccharides (e.g.d-arabinosev.l-arabinose) were confirmed. The uptake of metabolizable sugars (d-glucose,d-fructose) resulted in a gradual increase in protoplast (and cell) volume. The optical density of cells rose abruptly after some 20–60 min of incubation but this rise was not accompanied by a decrease in cell volume. The increase was prevented by 2,4-dinitrophenol but not by iodoacetic acid. The effect of temperature and of ionic strength of the medium was examined. Differences between protoplasts and intact cells in the utilization of sucrose were confirmed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Biely, P., Bauer, Š.:Metabolism of 2-deoxy-D-glucose by baker's yeast. I. Isolation and identification of phosphorylated esters of 2-deoxy-D-glucose. Coll. Czechosl. Chem. Commun. 32: 1588, 1967.

    CAS  Google Scholar 

  • Burger, M., Bacon, E. E., Bacon, J. S. D.:Some observations on the form and location of invertase in the yeast cell. Biochem. J. 78: 504, 1961.

    PubMed  CAS  Google Scholar 

  • Cirillo, V. P.:A comparison of sugar uptake by glucose grown and galactose grown baker's yeast. In: Symposium über Hefeprotoplasten (ed. by R. Müller), Jena 1965. Akademie-Verlag, Berlin, 1967.

    Google Scholar 

  • Kotyk, A.:Properties of the sugar carrier in baker's yeast. II. Specificity of transport. Fol. microbiol. 12: 121, 1967.

    CAS  Google Scholar 

  • Kotyk, A., Michaljaničová, D.:Properties of the sugar carrier in baker's yeast. IV. An asymmetric component of monosaccharide transport. Fol. microbiol. 13: 212, 1968.

    Article  CAS  Google Scholar 

  • LeFevre, P. G., LeFevre, M. E.:The mechanism of glucose transfer into and out the human red cell. J. gen. Physiol. 35: 891, 1952.

    PubMed  CAS  Google Scholar 

  • Nurminen, T., Oura, E., Suomalainen, H.:Preparation of protoplasts from baker's yeast. Suomen Kemistilehti B 38: 282, 1965.

    Google Scholar 

  • Ørskov, S. L.:Eine Methode zur fortlaufenden photographischen Aufzeichnung von Volumänderungen der roten Blutkörperchen. Biochem. Z. 279: 241, 1935.

    Google Scholar 

  • Volfová, O., Munk, V., Dostálek, M.:Lack of ability to oxidize hydrocarbons in protoplasts of Candida lipolytica. Experientia 23: 1005, 1967.

    Article  PubMed  Google Scholar 

  • Wilbrandt, W.:Zur Methodik der osmotischen Permeabilitätsmessung an Erythrocyten. Pflügers Arch. 250: 569, 1945.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory for Cell Membrane Transport, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4

    A. Kotyk & D. Michaljaničová

Authors
  1. A. Kotyk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Michaljaničová
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kotyk, A., Michaljaničová, D. Densitometry of yeast cells and protoplasts during sugar uptake. Folia Microbiol 14, 62–69 (1969). https://doi.org/10.1007/BF02869401

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02869401

Keywords

Search

Navigation