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The tomato test for eliminating spindle tuber from potato planting stock

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A lot of 792 tubers, of which about 20% were infected with the potato spindle tuber virus (PSTV), were subjected to greenhouse index tests, using tomato as a test plant. Tomato seedlings in the cotyledon stage were rubbed with the test material, either foliage or the surfaces of seed pieces cut from test tubers with a mellon-baller. Fourteen days later the test plants were inoculated with a severe strain of PSTV to detect the presence of mild strains. Tubers giving negative results were planted for propogation and those giving positive or doubtful results were planted in an observation plot. All potato plants were inspected visually and those in the observation plot were given an additional tomato test. The results indicate the validity and value of the tomato test for the elimination of PSTV from seed stocks before planting. The tuber test conducted in the winter was less efficient than the foliage test conducted in the spring. A modification of the test procedure is advocated in which two test plants are rubbed from each source, one to be challenged and one not. This increases the accuracy of the test, particularly during periods when symptoms of PSTV are poorly expressed.

Resumen

Un lote de 792 tubérculos, de los cuales más o menos 20% estaban infectados con el virus de papa ahusada (PSTV) fué sujeto de un ensayo de índice en invernadero, usando plantas de tomate como plantas de ensayo. Plantas germinadas de tomate con cotiledones fueron restregadas con el material de ensayo consistente ya sea de follaje, ya sea de las superficies de pedazos de papas cortadas de los tubérculos de ensayo con un cortador de melones. Catorce días más tarde las plantas de ensayo fueron inoculadas con una forma virulenta de PSTV para descubrir la presencia de formas blandas. Tubérculos que dieron resultados negativos fueron plantados para ser progados y aquellos que dieron resultados positivos o ambíguos fueron plantados en un lote de observación. Todas las plantas de papa fueron inspeccionadas visualmente y aquellas en el lote de observación recibieron un ensayo adicional con plantas de tomate. Los resultados indicaron la validez y la ventaja del ensayo por medio de plantas de tomate para la eliminación de PSTV de las papas antes de sembrar. El ensayo por medio de tubérculos conducido en invierno fue menos eficaz que el ensayo por medio del follaje hecho en primavera. Se recomienda una modificación del procedimiento de ensayo en la cual dos plantas de cada origen se restriegan y de las cuales una se examina y la otra no. Esto aumenta le exactitud del ensayo, especialmente durante los períodos cuando los síntomas de PSTV están pobremente expresados.

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Literature Cited

  1. Fernow, K. H. 1967. Tomato as a test plant for detecting mild strains of potato spindle tuber virus. Phytopathology 57: 1347–1352.

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  2. Singh, R. P. and R. H. Bagnall. 1968. Infectious necleic acid from host tissues infected with the potato spindle tuber virus. Phytopathology 58: 696–699.

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Fernow, K.H., Peterson, L.C. & Plaisted, R.L. The tomato test for eliminating spindle tuber from potato planting stock. American Potato Journal 46, 424–429 (1969). https://doi.org/10.1007/BF02868751

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