Abstract
The length of the carbon chain of the hydrocarbon substrate affects markedly the fatty acid composition in the cell lipids of the yeastCandida lipolytica 4-1. During cell growth onn-hexadecane dissolved in deparaffinated gas oil, direct incorporation of palmitic acid into lipids takes place. During growth onn-dodecane, on the other hand, an immediate synthesis and incorporation into oleic acid is observed. The cells contain only little lauric acid (maximum 11%). During fermentation of then-alkanes dissolved in deparaffinated gas oil which contains a mixture of isoalkanes, alkylated aromatic and cyclic hydrocarbons, free fatty acids accumulate in the oil phase. The fatty acid composition in the oil differs markedly according to the growth stage of cells. At the beginning of the logarithmic phase of growth, the fatty acid composition in the oil phase reflects the acid composition in the cell lipids, toward the end of cell growth, the cooxidation products of the isoalkanes accumulate. The aqueous phase contains lower fatty acids and cooxidation products of isoalkanes and of alkylated aromatic and alicyclic hydrocarbons.
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Dyatlovitskaya E. V., Greshnykh K. P., Bergelśon L. D.: Chemistry of lipids. IV. Lipids of yeast grown on normal alkanes. (In Russian).Prikl. Biokhim. Mikrobiol. 1, 613 (1965).
Dyatlovitskaya E. V., Greshnykh K. P., Zhdannikova E. I., Kozlova L. I., Bergelśon L. D.: Effect of pH on the composition of lipids ofCandida yeasts grown onn-alkanes. (In Russian)Prikl. Biokhim. Mikrobiol.,5, 511 (1969).
Greshnykh K. P., Grigoryan A. N., Dikanskaya E. M., Dyatlovitskaya E. V., Bergelśon L. D.: Effect of temperature and nitrogen source on lipid biosynthesis by yeast grown onn-alkanes. (in Russian).Mikrobiologiya 37, 251 (1968).
Klug M. J., Markowetz A. J.: Fermentation ofl-hexadecene byCandida lipolytica.Biotechnol. Bioeng. 11, 427 (1969).
Lideman L. S., Vorotilo C. P., Finkelshtein Z. I.: Synthesis of fatty acids by parafin-oxidizing yeastCandida lipolytica No. 670 (In Russian).Mikrobiol. Sintez 8, 21 (1969).
Lowery Ch. E.: Hydrocarbons as substrates for molds and yeasts.Diss. Abstr. 28, 161 (1967).
Melcalfe L. D., Schmitz A. A., Pelka J. R.: Rapid preparation of fatty acids ester from lipids for gas chromatographic analysis.Anal. Chem. 38, 514 (1966).
Miller T. L., Lie S., Johnson M. J.: Growth of a yeast of normal alkanes.Biotechnol. Bioeng. 6, 299 (1964).
Mizuno M., Shimosima Y., Iguchi T., Takeda I., Senoh S.: Fatty acid composition of hydrocarbon-assimilating yeast.Agr. Biol. Chem. 30, 506 (1966).
Pecka K., Havel Z., Mostecký J.: Study of methods of estimation of content and composition of residual hydrocarbons and lipids in yeast grown on hydrocarbon substrates.Research Report A-7-29/15c, College of Chemical Technology, Prague, 1969.
Pelechová J., Krumphanzl V., Uher J., Dyr J.: Assimilation of hydrocarbons. I. Proportion of fatty acids in the cell fat.Folia Microbiol. 16, 101 (1971).
Raděj Z., Antušek J., Halama D.: Oxidation ofn-alkanes by yeasts.Folia Microbiol. 16, 508 (1971).
Volfová O., Pecka K.: Production of fatty acids byCandida lipolytica during growth on puren-alkanes.Folia Microbiol. 16, 503 (1971).
Volfová O., Pecka K., Fenol Z.: The production of fatty acids during growth of the yeastCandida lipolytica onn-hexodecane andn-dodecane. Abstr. Commun. 7th Meet. Eur. Biochem. Soc. Varna, 20th Sept. 1971, p. 199.
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Part III. Oxidation and Utilization of Individual Pure Hydrocarbons—seeFolia Microbiol. 14,334 (1969).
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Volfová, O., Pecka, K. Cultivation ofCandida lipolytica 4-1 on hydrocarbons. Folia Microbiol 18, 286–299 (1973). https://doi.org/10.1007/BF02868045
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DOI: https://doi.org/10.1007/BF02868045