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Fluorescent antibody method

Quantitative Determination of 1-dimethylaminonaphthalene-5-sulphonic acid and protein in labelled γ-globulin

Метод флуоресцентных антител

Количественное определение 1-диметиламинонафтален-5-сульфоновой кислоты и белка в меченом γ-глобулине

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Summary

A spectrophotometric method for the quantitative determination of protein and 1-dimethylamininaphthalene-5-sulphonic acid in fluorescent antibodies was elaborated. It was shown that after exhaustive dialysis the conjugates do not contain free dye which would disturb the analysis. According to the conditions used during conjugation the average weight ratio of protein to fluorescent dye in the labelled γ-globulin was 76–245 corresponding to 3–9 molecules of dye bound to one molecule of globulin.

Abstract

Был разработан спектрофотометрический метод количественного определения белка и 1-диметиламинонафтален-5-сульфоновой кислоты в флюоресцентных антителах. Выяснилось, что после основательного диализа коньюгаты не содержат свободного красителя, который был бы помехой определений. В зависимости от условий коньюгации среднее отношение веса белка к флюоресцентному красителю в меченом γ-глобулине составляло 76–245, что отвечает 3–9 молекулам красителя, связанным с 1 молекулой глобулина.

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Authors and Affiliations

  1. Institute of Virology, Czechoslovak Academy of Sciences, Bratislava

    F. Sokol, L. Hána & P. Alberecht

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  1. F. Sokol
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  2. L. Hána
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  3. P. Alberecht
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Sokol, F., Hána, L. & Alberecht, P. Fluorescent antibody method. Folia Microbiol 6, 145–150 (1961). https://doi.org/10.1007/BF02864639

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