Abstract
Ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf and Rg1 were effectively isolated from ginseng root by preparative liquid chromatography (LC) on two PrepPAK-500/C18 cartridges in series and semipreparative LC on a μBondapak cabohydrate analysis column, a μBondapak C18 column or a μPorasil column. The identities of the isolated ginsenosides were confirmed by analytical high-performance liquid chromatography (HPLC) and infrared spectrophotometry. By this method large scale isolation of pure ginsenosides was efficiently accomplished.
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Sanada, S., Kondo, N., Shoji, J., Tanaka, O., and Shibata, S., Studies on the saponins of ginseng. I. Structures of ginsenoside Ro, Rb1, Rb2, Rc and Rd.Chem. Pharm. Bull.,22, 421 (1974).
Sanada, S., Kondo, N., Shoji, J., Tanaka, O., and Shibata, S., Studies on the saponins of ginseng. II. Structures of ginsenoside Re, Rf, Rg2.Chem. Pharm. Bull. 22, 2407 (1974).
Elyakov, G.B., Strigina, L.I., Uvarova, N.I., Vaskovsky, V.E., Dzizenko, A.K., and Kochetkov, H.K., Glycosides from ginseng roots.Tetrahedron Lett. 1964, 3591 (1964).
Elyakov, G.B., Uvarova, N.I., Krysina, V.P., and Antonik, L.M., Separation of the glycosides of ginseng on bio-gel p-2.Khimiya Prirodnkh Soedinenii 4, 54 (1968).
Oura, H., Hiai, S., Odaka, Y., and Yokozawa T.,J. Biochem. (Japan)77, 1057 (1975).
Kim, J.Y., and Staba, E.J., Plant biochemistry of ginseng saponins (I). Saponins and sapogenins from American ginseng plants.Proceedings of International Ginseng Symposium., The Central Research Institue Office of Monopoly, Seoul, Korea, p. 77 (1974).
Chen, S.E., and Staba, E.J., American ginseng. I. Large scale isolation of ginsenosides from leaves and stems.Lloydia 41, 361 (1978).
Nagasawa, T., Yokozawa, T., Nishino, Y., and Oura, H., Application of high-performance liquid chromatography to the isolation of ginsenoside Rb1, Rb2, Rc, Rd, Re, and Rg1 from ginseng saponins.Chem. Pharm. Bull. 28, 2059 (1980).
Choi, J.H., Kim, W.J., Hong, S.K., Oh, S.K., and Oura, H., High-performance Liquid Chromatographic isolation of ginsenoside Rf, Rg2 and Rh1.J. Korean Agr. Chem. Soc. 23, 206 (1980).
Sticher, O., and Soldati, F., HPLC separation and quantitative determination of ginsenosides fromPanax ginseng, Panax quinquefolium and from ginseng drug preparations (I).Planta Medica 36, 30 (1979).
Soldati, F., and Sticher, O., HPLC separation and quantitative determination of ginsenosides fromPanax ginseng, Panax quinquefolium and from ginseng drug preparation (II).Planta Medica 38, 348 (1980).
Hong, S.K., Park, E.K., Lee, C.Y. and Kim, M.U., High-performance liquid chromatographic determination of ginseng saponins.Yakhak Hoeji 23, 181 (1979).
Besso, H., Saruwatari, Y., Fuamura, K., Kunihiro, K., Fuwa, T., and Tanaka, O., High-performance liquid chromatographic determination of ginseng saponin by ultraviolet derivatisation.Planta Medica 37, 226 (1979).
Han, B.H., Studies on the preparation of ginseng pure saponins.Ginseng Shihum Yunku Yongyuk Bokoseo (Report), section 7, Korea Ginseng Research Institute, Seoul, 1978.
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Paik, N.H., Park, M.K., Choi, K.J. et al. Isolation of ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf and Rg1 from ginseng root by high performance liquid chromatography. Arch. Pharm. Res. 5, 7–12 (1982). https://doi.org/10.1007/BF02856357
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DOI: https://doi.org/10.1007/BF02856357