Abstract
Sodium bisulfite, cysteine, dithiothreitol, and sodium diethyldithiocarbamate (Na Dieca) inhibit enzymatic blackening in potatoes by initially inhibiting the oxidation of tyrosine by polyphenol oxidase. Sodium bisulfite and Na Dieca also cause enzyme inactivation. Although cysteine and dithiothreitol inhibit tyrosine oxidation for a period of time, they do not appear to inactivate the enzyme. Dithiothreitol is a much more effective inhibitor of tyrosine oxidation than cysteine.
Ascorbic and dihydroxyfumaric acids inhibit enzymatic blackening by reducing the dopaquinone to dopa. When the reducing agents are completely oxidized, blackening can occur.
Resumen
Bisulfito de Sodio, cisteína, ditiotreitol y dietil ditiocarbonato (Dieca de Sodio) inhiben el obscurecimiento (necrosis) enzimática en papas a causa de la inhibición en la oxidación de la tirosina causada por la oxidasa poliferiótica. El bisulfito de Sodio y el Dieca de Sodio también causan inactivación de la enzima. Aunque la cisteína y el ditiotreitol inhiben la oxidación de la tirosina por un cierto período, aparentemente no inactivan la enzima. El Ditiotreitol es un inhibidor much más efectivo de la oxidación de la tirosina que la cisteína.
Los ácidos ascórbico y dihidroxifumárico disminuyen el obscurecimiento enzimático a través de su acción de reducir la dopaquinona a dopa. Cuando los agentes reductores son oxidados totalmente se produce el obscurecimiento del tejido.
Similar content being viewed by others
Literature Cited
Amla, B. L. and F. J. Francis. 1961. Effect of pH of dipping solution on the quality of pre-peeled potatoes. Am Potato J 38 (4), 121–130.
Cecil, R. 1963. Intramolecular bonds in proteins. I. Role of sulfur in proteins.In “The Proteins, Vol. I”, H. Neurath, ed., Academic Press. N.Y. pp. 379–476.
Embs, R. J. and P. Markakis. 1965. The mechanism of sulfite inhibition of browning caused by polyphenol oxidase. J Food Sci 30, 753–758.
Joslyn, M. A. and J. D. Ponting. 1951. Enzyme catalyzed oxidative browning of fruit products. Adv Food Res III. pp. 1–46.
Lerner, A. B. 1953. Metabolism of phenylalanine and tyrosine. Adv in Enzymol 14, 73–128.
Makower, R. U. 1964. Adensine triphosphate (ATP)-induced inhibition of potato browning. Effect of ascorbic acid and of reducing substances. Plant Physiol 39 (4): 520–2.
Mathew, A. G. and H. A. B. Parpia. 1971. Food browning as a polphenol reaction. Adv in Food Res 19, 75–145.
Muneta, P. 1966. Bisulfite inhibition of enzymatic blackening caused by tyrosine oxidation. Am Potato J 49:397–402.
Muneta, P. 1967. Possible interaction of chlorogenic acid, tyrosine, and bisulfite in enzymatic blackening of potatoes. Am Potato J 44:236–40.
Muneta, P. and Wlradt. 1968. Cysteine inhibition of enzymatic blackening with polyphenol oxidase from potatoes. J. Food Sci 33 (6) 606–8.
Muneta, P. and H. Wang. 1977. Influence of pH and bisulfite on the enzymatic blackening reaction in potatoes. Am Potato J 54:73–81.
Ponting, J. D. 1960. The control of enzymatic browning of fruits.In Food Enzymes. ed. H. W. Schultz. The Avi Publ. Co. Inc., Westport, Conn. pp. 105–124.
Author information
Authors and Affiliations
Additional information
Published with the approval of the Director of the Idaho Agricultural Experiment Station as Research Paper No. 8055.
Rights and permissions
About this article
Cite this article
Muneta, P. Comparisons of Inhibitors of Tyrosine Oxidation in the enzymatic blackening of potatoes. American Potato Journal 58, 85–92 (1981). https://doi.org/10.1007/BF02854377
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02854377