Abstract
Plants free of potato virus S were obtained from infected nodal segments cultured on agar solidified medium containing antiviral compounds. After two to 12 weeks, the uppermost node of the resultant plant was recovered, cultured and tested by Enzyme Linked Immunosorbent Assay. The compounds 6-azauracil, 6-azathymine and 5-fluorouracil were ineffective and melamine (2,4,6-triamino-s-triazine) and 2-thiouracil eliminated potato virus S (PVS) sporadically but ribavirin (l-β-D-ribofuranosyl-1H-l, 2,4-triazole-3-carboxamide) gave PVS eradication rates of 10 percent or more and was the most effective antiviral tested in this system. The method is technically simple, rapid and effective. It has been tested with over 100 varieties and has also proven useful in eliminating potato viruses X, Y, and LR.
Compendio
Se obtuvieron plantas libres del virus S, de segmentas nodales infectados cultivados sobre medio solidificado de agar conteniendo compuestos antivirales. Después de dos a 12 semanas, se recuperó el nudo superior de la planta resultante al que se cultivó y probó luego utilizando la técnica ELISA. Los compuestos 6-azauracilo, 6-azatimina y 5-fluorouracilo no fueron efectivos y melamina (2, 4, 6-triamino-s-triazina) y 2-tiouracilo eliminaron al virus S de la papa (PVS) esporádicamente, pero ribavirin (l-β-D-ribofuranosilo-1H-1,2,4-triazole-3-carboxamida) dieron tasas de erradicación del PVS de 10% o más y fue el antiviral más efectivo de los probados con este sistema. El método es técnicamente simple, rápido y efectivo. Ha sido probado con más de 100 variedades y se ha encontrado también útil para eliminar los virus X, Y y LR de la papa.
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Conrad, P.L. Potato virus S-free plants obtained using antiviral compounds and nodal segment culture of potato. American Potato Journal 68, 507–513 (1991). https://doi.org/10.1007/BF02853767
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DOI: https://doi.org/10.1007/BF02853767