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Expression of a pea disease resistance response gene in the potato cultivar Shepody

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Abstract

The coding sequence from the pea disease resistance response gene 49 (DRR49 gene), was transferred into the potato (Solanum tuberosum L.) cultivar Shepody viaAgrobacterium co-cultivation. Potato leaf explants were co-cultivated withAgrobacterium tumefaciens harboring a binary vector containing the coding sequence from the DRR49 gene under the control of the 35S promoter from cauliflower mosaic virus (CaMV). Transformed potato plants were selected by their resistance to kanamycin. The insertion of foreign DNAs was detected by polymerase chain reaction (PCR) and Southern blot analysis. The expression of DRR49 coding sequence was confirmed via the detection of the corresponding mRNA in northern blot analysis. Field pathogenicity tests indicated that transgenic plants expressing the pea DRR49 mRNA have higher tuber yields than the control plants when grown in PED (potato early dying) infested soil.

Compendio

La secuencia de codificatión del gen de respuesta 49 (gen DRR49) de la resistencia a una enfermedad de la arveja, fue transferida al cultivar de papa (Solanum tuberosum L.) Shepody por intermedio de un cultivo conjunto conAgrobacterium. Cortes de hoja de papa fueron cultivados conjuntamente conAgrobacerium tumefaciens portando un vector binario conteniendo la secuencia de codificación del gen DRR49 bajo el control del promoter 35S del virus del mosaico de la coliflor (CaMV). Las plantas transformadas de papa fueron seleccionadas por su resistencia a la kanamicina. Se detecto la insertión de DNAs importados por medio de la reacción en cadena de polimerasa (PCR) y el análisis del DNA en membranas de nitrocelulosa (“Southern blot analysis”). La expresión de la secuencia de codificatión del DRR49 se confirmó mediante la detection del correspondiente mRNA en análisis sobre membranas de nitrocelulosa (“Northern blot análisis”). Pruebas de patogenecidad en el camp indicaron que las plantas transgénicas expresando el DRR49 raRNA de la arveja tienen rendimientos en tubérculos mayores que las plantas testigo cuando crecen en suelos infestados con muerte prematura de la papa (PED = potato early dying).

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Abbreviations

MS:

Murashige and Skoog

NAA:

naphthaleneacetic acid

CAT:

chloramphenicol acetyltransferase

DRR49 gene:

disease resistance response gene 49

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Author notes

  1. Chin C. Chiang

    Present address: Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, PCTB714, 725 N. Wolfe, 21205, Baltimore, MD

Authors and Affiliations

  1. Department of Plant Pathology, Washington State University, 99164-6430, Pullman, WA

    Ming-Mei Chang & Lee A. Hadwiger

  2. USDA-ARS, Washington Sate University Irrigated Agriculture Research and Extension Center, 99350-9687, Prosser, WA

    Mark W. Martin

Authors
  1. Ming-Mei Chang
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  2. Chin C. Chiang
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  3. Mark W. Martin
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  4. Lee A. Hadwiger
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Chang, MM., Chiang, C.C., Martin, M.W. et al. Expression of a pea disease resistance response gene in the potato cultivar Shepody. American Potato Journal 70, 635–647 (1993). https://doi.org/10.1007/BF02849153

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