Abstract
Study on the transient expression of GUS gene at different growing stage ofChlorella ellipsoidea using high velocity microprojectiles, the effects of osmosis, the distance between microprojectile and target cell, bombardment times, are reported in this paper. The results showed thatC. ellipsoidea in exponential phase has higer level of transient expression and that treatment with osmosis can improve the GUS transient expression notably. The effect of distance or bombardment times was not observed.
Similar content being viewed by others
References
Cheney, D. P., 1982. Progress in protoplast fusion and gene transfer in red alga. Abstracts, 16th International Seaweed Symposium, Brittany, France, No. 61.
Hall, L. M., Taylor, K. B., Jones, D. D. et al., 1993. Expression of a foreign gene inChlamydomonas reinhardtii.Gene 124(1): 75–81.
Hua Rucheng, 1986. Culture and Utilization of Unicellular Algae. China Agricultural Press, Beijing. p. 272–274. (in Chinese)
Jarvis, E. E., Brown, L. M., 1991. Transient expression of firefly luciferase in protoplast of the green algaChlorella elliposoidea.Curr. Genet. 19: 317–321.
Jefferson, R. A., Kavanagh, T. A., Bevan, M. V., 1987.GUS fusion β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.EMBO J. 6(30): 3901–3907.
Kindle, K. L., 1990. High-frequency nuclear transformation ofChlamydomonas reinhardtii.Proc. Natl. Acad. Sci. USA 87: 1228–1232.
Kindle, K. L., Mayfield, S. P., 1990. Stable nuclear transformation ofChlamydomonas reinhardtii by using aC. reinhardtii gene as the selectable marker.Proc. Natl. Acad. Sci. USA 87: 2087–2091.
Qin Song, Zhang Jian, Li Wenbin et al., 1994. Transient expression ofGUS gene in phaeophytes using biolistic particle delivery system.Oceanologia et Limnologia Sinica.25(4): 353–357. (in Chinese, with English abstract)
Rochaix, J. D., Dillewijn, J. Van, 1982. Transformation of the green algaeChlamydomonas reinhardtii with yeast DNA.Nature 296: 70–72.
Takashi Yamada, Kenji Sakaguchi, 1981. Protoplast induction inChlorella species.Agric. Biol. Chem. 45(8): 1905–1909.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ying, C., Wen-bin, L., Qin-hua, B. et al. Study on transient expression of gus gene inChlorelia ellipsoidea (Chlorophyta) by using biolistic particle delivery system. Chin. J. Ocean. Limnol. 16 (Suppl 1), 47–49 (1998). https://doi.org/10.1007/BF02849079
Issue Date:
DOI: https://doi.org/10.1007/BF02849079