Abstract
Chondrus is an economically important red algae widely used for food and biochemical purpose. It early development is crucial for the culture and seedling propagation. We chose tetraspores and carpospores ofChondrus ocellatus as examples for experiment of the culture, induction and release in laboratory condition, aiming to understand early development ofC. ocellatus and to apply in seedling production. MatureC. ocellatus were collected in Qingdao, China, from Nov. to Dec. 2004. After the gametophyte and tetrasporophyte were brushed and washed with sterilized seawater, the algal materials were treated in 1.5% KI for 20 min, then were dried for 1h to stimulate the releasing of spores. After the spores released overnight, it were cultured in PES medium, incubated at 18 °C, 10±2 μmol/(m2·s1) in 12∶12h (light: dark). The observation and recording under microscope were carried out. Continuous observation of the early development showed that both tetraspore and carpospore are similar to each other. In general, three stages of the early development were shown being division, discoid crust and seedling stages. To the division stage, the most obvious feature was the increasing of cell number; during the discoid crust stage, the discoid crust had a three-dimensional axis, and it began to differentiate into two types of cells: the basal cells and the apical cells; and to the seedling stage, several protuberances-like appeared on the discoid crusts and formed juvenile seedlings. Carpospores and tetraspores exhibited a similar development process that included division stage, discoid crust stage and seedling stage.
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References
Anderson, N. S. and D. A. Rees, 1966. The repeating structure of polysaccharide sulphate from red seaweeds.In: Young, E. G. and J. McLachlar, eds., Proc. 5th Int. Seaweed Symp. Pergamon Press, London, pp. 243–249.
Burns, R. L. and A. C. Mathieson, 1972. Ecological Studies of Economic Red Algae. II. Culture studies ofChondrus crispus Stackhouse andGigartina stellata (Stackhouse) batters.J. Exper. Mar. Bio. Ecol. 8(1): 1–6.
Carrington, E. and S. P. Grace, 2001. Life history phase and the biomechanical properties of the red algaChondrus crispus (Rhodophyta).J. Phycol. 37: 699–704.
Chen, M. Q. and G. Z. Ren, 1985. The development process of sporelings ofGracilaria verrucosa (Hudson) Papenfuss.Oceanologia et Limnologia Sinica.16(3): 181–187. (in Chinese)
Chopin, T., 1986. The red algaChondrus crispus Stackhouse (Irish moss) and carrageenans: a review.Can. Tech. Rep. Fish. Aquat. Sci. 1 514: 1–69.
Chopin, T. and B. T. Wagey, 1999. Factorial study of the effects of phosphorus and nitrogen enrichments on nutrient and carrageenan content inChondrus crispus (Rhodophyceae) and on residual nutrient concentration in seawater.Botanica Marina.42: 23–31.
Craigie, J. S. and J. D. Pringle, 1978. Spatial distribution of tetrasporophytes and gametophytes in four maritime populations ofChondrus crispus.Can. J. Bot. 56: 2 910–2 914.
Hori, T., 1993. An Illustrated Atlas of the Life History of Algae. Vol. 2. Brown and Red Algae. Uchdia Rokakuho Publishing Co., Ltd. Tokyo. Japan.
Jones, W. E., 1956. Effect of spore coalescence in the early development ofGracilaria verrucosa (Huds.) Papenfuss.Nature 178: 426–427.
Komiyama, T. and M. Sasamoto, 1957. Studies on the propagation ofGracilaria verrucosa (Huds.) Papenfuss I. On the settling of the spores and development of the early stage.Report of the Investigations on the Ariake Sea.4: 25–34.
Gutierrez, L. M. and C. Fernandez, 1992. Water motion and morphology inChondrus crispus (Rhodophyta).J. Phycol. 28: 156–162.
Mathieson, A. C. and E. Tveter, 1975. Carrageenan ecology ofChondrus crispus Stackhouse.Aquatic Botany.1: 25–43.
Santelices, B., M. Hormazabal, J. Correa and V. Flores, 2004. The fate of overgrown germlings in coalescing Rhodophyta.Phycologia 43(4): 346–352.
Stancioff, D. and N. F. Stanley, 1969. Infrared and chemical studies on algal polysaccharides.In: Margalef, R. ed., Proc. 9th Int. Seaweed Symp., Santiage de Compostela, Spain. Subsecretaria de la Marina Mercante, Madrid, pp. 595–609.
Noda, H., 1990. Antitumor activity of marine algae.Proc Int. Seaweed Symp. 13: 577–584.
Pacheco-Ruiz, I., J. A. Zertuche-Gonzalez and J. Espinoza-Avalos, 2005. The role of secondary attachments discs in the survival ofChondracanthus squarrulosu (Gigartinales, Rhodophyta).Phycologia.44(6): 629–631.
Provasoli, L., 1968. Media and prospects for the cultivation of marine algae.In: Watanabe A. and Hattori A. eds., Culture and Collections of Algae. Japan. Soc. Plant Physiol., Tokyo, Proc., pp. 63–75.
Zhou, G. F., Y. P. Sun, H. Xin, Y. N. Zhang, Z. E. Li and Z. H. Xu, 2004. In vivo antitumor and immunomodulation activities of different molecular weight lambdacarrageenans fromChondrus ocellatus.Pharmacological Research 50(1): 47–53.
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Supported by NSFC (No. 40376049) and Shandong Agricultural Seed Stock Breeding Project.
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Aihua, W., Jicheng, W. & Delin, D. Early development ofChondrus ocellatus holm (Gigartinaceae, Rhodophyta). Chin. J. Ocean. Limnol. 24, 129–133 (2006). https://doi.org/10.1007/BF02842811
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DOI: https://doi.org/10.1007/BF02842811