Summary
The protective effect of carvedilol on abnormality of L-type calcium current induced by oxygen free radical in single guinea pig ventricular myocytes was studied. Whole-cell patch clamp technique was used to study the effect of H2O2 (0.5 mmol/L) on L-type calcium current in single guinea pig ventricular myocytes and the action of pretreatment with carvedilol (0.5 μmol/L). 0.5 μmol/L carvedilol had no significant effect onI Ca.L and its channel dynamics. In the presence of 0.5 mmol/L H2O2, peak current ofI Ca.L was reduced significantly (P<0.001), theI-V curve ofI Ca.L was shifted upward, steady-state activation curve and steady-state deactivation curve ofI Ca.L were shifted left and recovery time ofI Ca.L was delayed significantly (P<0.001). 0.5 μmol/L carvedilol significantly alleviated the inhibitory effect of H2O2 onI Ca.L as compared with that in H2O2. It was concluded that carvedilol could alleviate the abnormality of L-type calcium current induced by oxygen free radical in cardiomyocytes. It shows partly the possible mechanism of the special availability of carvedilol in chronig heart failure.
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References
Book W M. Carvedilol: a nonselective beta, blocking agent with antioxidant properties. Congest Heart Fail, 2002, 8(3):173
Poole-Wilson P A, Swedberg K, Cleland J Get al. Comparison of carvedilol and metoprolol on clinical outcomes in patients with chronic heart failure in the Carvedilol or Metoprolol European Trial (COMET): randomised controlled trial. Lancet, 2003, 362(9377):7
Ferrari R, Agnoletti L, Comini Let al. Oxidative stress during myocardial ischaemia and heart failure. Eur. Heart J, 1998, 19(Suppl B):B2
Mak S, Newton G E. The oxidative stress hypothesis of congestive heart failure: radical thoughts. Chest, 2001, 120(6):2035
Mitra R, Morad M. A uniform enzymatic method for dissociation of myocytes from hearts and stomachs of vertebrates. Am J Physiol, 1985, 249(2):H1056
Dargie H J. Beta blockers in heart failure. Lancet, 2003, 362(9377):2
Beta-Blocker Evaluation of Survival Trial Investigators. A trial of the beta-blocker bucindolol in patients with advanced chronic heart failure. N Engl J Med, 2001, 344(22):1659
Stoschitzky K, Koshucharova G, Zweiker Ret al. Differing beta-blocking effects of carvedilol and metoprolol. Eur J Heart Fail, 2001, 3(3):343
Kalra J, Prasad K. Oxygen free radicals and cardiac depression. Clin Biochem, 1994, 27(3):163
Cheng J, Niwa R, Kamiya Ket al. Carvedilol blocks the repolarizing K+ currents and the L-type Ca2+ current in rabbit ventricular myocytes. Eur J Pharmacol, 1999, 376(1–2):189
Flesch M, Maack C, Cremers Bet al. Effect of β-blockers on free radical-induced cardiac contractile dysfunction. Circulation, 1999, 100(4):346
Feuerstein G, Yue T L, Ma Xet al. Novel mechanisms in the treatment of heart failure: inhibition of oxygen radicals and apoptosis by carvedilol. Prog Cardiovasc Dis, 1998, 41(Suppl 1):17
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LIU Nian, male, born in 1973, Doctor in charge
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Nian, L., Ronghui, Y., Yanfel, R. et al. Protective effect of carvedilol on abnormality of L-type calcium current induced by oxygen free radical in cardiomyocytes. Current Medical Science 24, 433–436 (2004). https://doi.org/10.1007/BF02831101
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DOI: https://doi.org/10.1007/BF02831101