Summary
In order to investigate the neurotoxicity of lipopolysaccharide (LPS) on the dopaminergic neurons of substantia nigra and the pathogenesis of Parkinson disease. LPS was stereotaxically infused into substantia nigra (SN). At different dosages and different time points with 5 μg LPS, the damage of the dopaminergic neurons in SN was observed by using tyrosine-hydroxylase (TH) immunohistochemical staining. The results showed that 14 days after injection of 0.1 μg to 10 μg LPS into the rat SN, TH-positive (TH+) neurons in the SN were decreased by 5%, 15%, 20%, 45%, 96% and 99% respectively. After injection of 5 μg LPS, as compared with the control groups, TH+ neurons began to decrease at 3rd day and obviously decrease at 14th day, only 5% of total cells, and almost disappeared 30 days later. The results suggested that LPS could induce the degeneration of dopaminergic neurons in the SN in a dose- and time-dependent manner.
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LI Gang, male, born in 1974, Doctor in Charge
This Project was supported by a grant from National Natural Sciences Foundation of China (No. 30170334).
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Gang, L., Shenggang, S., Xuebing, C. et al. LPS-induced degeneration of dopaminergic neurons of substantia nigra in rats. Current Medical Science 24, 83–86 (2004). https://doi.org/10.1007/BF02830714
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DOI: https://doi.org/10.1007/BF02830714