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Experimental study on meiotic spindles and chromosomes of mouse mature (M II) stage oocytes under laser canning confocal microscopy

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Summary

Taking the mouse as a model, the experimental method of observing the morphology of meiotic spindles and chromosomes in mature oocytes were investigated in order to evaluate the effects of various interventions on the quality of oocytes accurately and rapidly. Laser scanning confocal microscope (LSCM) was used to examine the meiotic spindles and chromosomes by the technologies of optical section and three-dimensional (3D) image reconstruction. The results showed that the configurations of meiotic spindles and chromosomes could be observed clearly by LSCM. The normal rate of meiotic spindles and chromosomes was 82% and 86% respectively. It was concluded that the LSCM was a valid instrument to observe the meiotic spindles and chromosomes of mature oocytes and could be used as a valid method to evaluate the quality of M II oocytes.

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Authors and Affiliations

  1. Reproductive Medical Center, Department of Obstetrics and Gynecology, Tongji Hopital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China

    Wang Yanlin & Zhu Guijin

  2. Molecular Medical Center, Tongji Hopital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China

    Li Xiaolan

  3. Department of Surgery, Public Health Clinic of Dudian Town, 256657, Binzhou, China

    Li Xia

Authors
  1. Wang Yanlin
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  2. Zhu Guijin
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  3. Li Xia
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  4. Li Xiaolan
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WANG Yanlin, female, born in 1972, M. D., Ph. D., Doctor in Charge

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Yanlin, W., Guijin, Z., Xia, L. et al. Experimental study on meiotic spindles and chromosomes of mouse mature (M II) stage oocytes under laser canning confocal microscopy. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 26, 353–355 (2006). https://doi.org/10.1007/BF02829574

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  • DOI: https://doi.org/10.1007/BF02829574

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