Summary
To investigate whether apoptosis is associated with cell adhesion in bronchial epithelium, and whether it contributes to the kinetics of injury and repair of surface epithelia, this study was performed for E-cadherin expression by using immunohistochemistry technique and for apoptosis by TUNEL method. An animal model of smoking was used for this study. The results showed that epithelial cells with membrane anchored E-cadherin decreased remarkably at several time points during 6 months of exposure to smoke (P<0.01) and then restored to normal level. This fluctuation was associated exclusively with the alteration in number of apoptotic cells (P<0.01). There was no significant difference in activation of nuclear transcription factor NF-kappa B among groups (P>0.05). All these suggested that apoptosis is associated with E-cadherin expression in bronchial epithelium of smoking mouse.
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References
Peluso J J, Pappalardo A, Fernandez G. E-cadherin-mediated cell contact prevents apoptosis of spontaneously immortalized granulosa cells by regulating Akt kinase activity. Biol Reprod, 2001, 64(4):1183
Vallorosi C J, Day K C, Zhao Xet al. Truncation of the beta-catenin binding domain of E-cadherin precedes epithelial apoptosis during prostate and mammary involution. J Biol Chem, 2000, 275(5):3328
Sasaki C Y, Lin H C, Passaniti A. Expression of E-cadherin reduces bcl-2 expression and increases sensitivity to etoposide-induced apoptosis. Int J Cancer, 2000, 86(5): 660
Ozawa M, Kemler R. Altered cell adhesion activity by prevanadate due to the dissociation of alpha-catenin from the E-cadherin-catenin complex. J Bio Chem., 1998, 273: 6166
Willert K, Nusse R. Beta-catenin: a key mediator of wnt signaling. Curr Opin Genet Dev, 1998, 8:95
Shinichi K, Masashi S, Fuloshi Tet al. Evidence that de novo protein synthesis is dispensable for anti-apoptosis effects of NF-kappa B. Oncogene, 2000, 19:2233
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LIU Lifang, female, born in 1971, Postgraduate
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Lifang, L., Yonghui, Y., Fang, L. et al. Relationship between apoptosis and E-cadherin expression in bronchial epithelium of smoking mouse. Current Medical Science 23, 216–218 (2003). https://doi.org/10.1007/BF02829495
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DOI: https://doi.org/10.1007/BF02829495