Skip to main content
SpringerLink
Log in

Gene expression of adiponectin and adiponectin receptor 1 in type 2 diabetic rats and the relationship with the parameters of glucose and lipid metabolism

  • Published:
Current Medical Science Aims and scope Submit manuscript

Summary

In order to confirm whether the mRNA levels of adiponectin in adipose tissue and mRNA levels of AdipoR1 in the skeletal muscles were correlated with the serum parameters of glucose and lipid metabolism and to clarify the regulation of adiponectin receptor gene expression in diabetic states, serum adiponectin, mRNA levels of adiponectin in adipose tissue and mRNA levels of AdipoR1 in the skeletal muscles were examined in type 2 diabetic rats. The model of type 2 diabetes was prepared by feeding high fat diet and injecting low dosage of streptozotocin (STZ). The diabetic rats were screened out by oral glucose tolerance test. One group of type 2 diabetic rats received rosiglitazone. The serum adiponectin concentration was detected by using ELISA and mRNA levels were examined by RT-PCR. The serum adiponectin levels and mRNA levels of adiponectin in adipose tissue of type 2 diabetic rats were significantly decreased as compared with the normal control rats (P<0.05,P<0.01 respectively). No siglificant changes were observed in the expression of adiponectin receptor 1 in the skeletal muscle of type 2 diabetic rats. The mRNA levels of adiponectin in adipose tissue were reversely correlated with serum insulin (r=−0.66,P<0.05), triglyceride (r=−0.58,P<0.05), cholesterol (r=−0.49,P<0.05), interleukin-6 (r=−0.49,P<0.05) and tumor necrosis factor (r=−0.43,P<0.05). The expression of adiponectin receptors was not altered in the skeletal muscle of Type 2 diabetic rats. The decreased serum adiponectin was caused by the decreased expression of adiponectin mRNA in adipose tissue rather than the adiponectin receptors in the skeletal muscle, which could be improved by rosiglitazone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Yamauchi T, Kamon J, Ito Yet al. Cloning of adiponectin receptors that mediate antidiabetic metabolic effects. Nature, 2003,423(6941):762

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Pascoe W S, Storlien L H, Inducement by fat feeding of basal hyperglycemia in rats with abnormal beta-cell function. Model for study of etiology and pathogenesis of NIDDM. Diabetes, 1990,39:226

    Article  CAS  PubMed  Google Scholar 

  3. Frühbeck G, Gósmez-Ambrosi J, Muruzábal F Jet al. The adipocyte: a model for integration of endocrine and metabolic signaling in energy metabolism regulation. Am J Physiol Endocrinol Metab, 2001,280:E827

    Article  PubMed  Google Scholar 

  4. Hu E, Liang P, Spiegelman B M. AdipoQ is a novel adipose-specific gene dysregulated in obesity. J Biol Chem, 1996,271(18):10–697

    Article  Google Scholar 

  5. Yamauchi T, Kamon J, Waki Het al. The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med, 2001,7(8):941

    Article  CAS  PubMed  Google Scholar 

  6. Kubota N, Terauchi Y, Yamauchi Tet al. Disruption of adiponectin causes insulin resistance and neointimal formation. J Biol Chem, 2002, 277(29):25–863

    Article  Google Scholar 

  7. Yamauchi T, Kamon J, Minokoshi Yet al. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase, Nat Med, 2002,8(11):1288

    Article  CAS  PubMed  Google Scholar 

  8. Yamauchi T, Kamon J, Waki Het al. Globular adiponectin protected ob/ob mice from diabetes and ApoE-deficient mice from atherosclerosis. J Biol Chem, 2003,278(4):2461

    Article  CAS  PubMed  Google Scholar 

  9. Hoffstedt J, Arvidsson E, Sjolin Eet al. Adipose tissue adiponectin production and adiponectin serum concentration in human obesity and insulin resistance. J Clin Endocrinol Metab, 2004, 89(3):1391

    Article  CAS  PubMed  Google Scholar 

  10. Debard C, Laville M, Berbe Vet al. Expression of key genes of fatty acid oxidation, including adiponectin receptors, in skeletal muscle of type 2 diabetic patients. Diabetologia, 2004,47(5):917

    Article  CAS  PubMed  Google Scholar 

  11. Tsuchida A, Yamauchi T, Ito Yet al. Insulin/Foxol pathway regulates expression levels of adiponectin receptors and adiponectin sensitivity. J Biol Chem, 2004, 279(29):30–817

    Article  Google Scholar 

  12. Staiger H, Kaltenbach S, Staiger Ket al. Expression of adiponectin receptor mRNA in human skeletal muscle cells is related to in vivo parameters of glucose and lipid metabolism. Diabetes, 2004,53(9):2195

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China

    Yao Hui, Ling Hanhua, Wang Hongwei, Zhang Longjiang, Huang Xiaoyan & Xia Zhi

Authors
  1. Yao Hui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ling Hanhua
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wang Hongwei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhang Longjiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Huang Xiaoyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xia Zhi
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

YAO Hui, female, born in 1965, Associate Professor

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hui, Y., Hanhua, L., Hongwei, W. et al. Gene expression of adiponectin and adiponectin receptor 1 in type 2 diabetic rats and the relationship with the parameters of glucose and lipid metabolism. Current Medical Science 25, 285–288 (2005). https://doi.org/10.1007/BF02828144

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02828144

Key words

Search

Navigation