Summary
Ripe and immature seeds ofOrchis papilionacea (method I and II, respectively) cultured on modified double strength Curtis medium were assayed for minituber production. Ripe seed germination both on solid and in liquid medium was low and the protocorms obtained developed into white calluses. Germination increased from, 9 to 33% when immature seed suspension culture was used. Protocorms obtained in suspension culture under light developed into minitubers, whereas those obtained on solid media developed into callus. A 30 s ultrasonication of immature seeds 1 wk after suspension, culture initiation further enhanced germination and minituber production. Minitubers had to be transferred and embedded in solid regeneration medium for normal growth.
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Pedroso, M.C., Pais, M.S. Minituber production from immature seed suspension culture ofOrchis papilionacea . In Vitro Cell.Dev.Biol.–Plant 28, 183–186 (1992). https://doi.org/10.1007/BF02823314
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DOI: https://doi.org/10.1007/BF02823314