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Sepharose spin column chromatography

A fast, nontoxic replacement for phenol: Chloroform extraction/ethanol precipitation

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References

  1. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989)Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  2. Holmes, D. S. and Quigley, M. (1981) A rapid boiling method for the preparation of bacterial plasmids.Anal. Biochem. 114, 193–197.

    Article  PubMed  CAS  Google Scholar 

  3. Deng, W. P. and Nickoloff, J. A. (1992) Site-directed mutagenesis of virtually any plasmid by eliminating a unique site.Anal. Biochem. 200, 81–88.

    Article  PubMed  CAS  Google Scholar 

  4. Fujimura, H. and Sakuma, Y. (1993) Simplified isolation of chromosomal and plasmid DNA from yeasts.BioTechniques 14, 538,539.

    PubMed  CAS  Google Scholar 

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  1. Department of Cancer Biology, Harvard School of Public Health, 665 Huntington Avenue, 02115, Boston, MA

    Jac A. Nickoloff

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  1. Jac A. Nickoloff
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Nickoloff, J.A. Sepharose spin column chromatography. Mol Biotechnol 1, 105–108 (1994). https://doi.org/10.1007/BF02821513

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  • DOI: https://doi.org/10.1007/BF02821513

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