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Substrate dependent bioaccumulation of cadmium by growing yeastCandida utilis

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Abstract

The ability of growing yeastCandida utilis to accumulate cadmium ions from the cultivation broth depends on the carbon source used; xylose and glucose were tested here. For the two substrates, the course of cadmium bioaccumulation and total uptake were quite different. With glucose the maximum of bioaccumulation was about ten times higher than with xylose. The data were consistent with a simple mathematical model of process dynamics which assumes a Freudlich isotherm to describe the biosorption equilibrium and first-order dynamics to simulated the transient state.

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Abbreviations

c :

cadmium ion concentration in the solution, mg/L

k F :

proportionality constant in Freudlich sorption isotherm, L/g dry matter

K s :

constant characterizing the substrate limitation, g/L

n :

exponent in Freundlich sorption isotherm

q :

cadmium content in the biomass, mg Cd per g dry matter

S :

substrate concentration, g/L

t :

current time, h

X :

biomass concentration, g dry matter per L

Y x/s :

yield coefficient

μ:

specific growth rate, 1/h

τ:

time constant of bioaccumulation, h

Indices :

e equilibrium, 0 initial, max maximum

References

  • Babich H., Devanas M.A., Stotzky G.: The mediation of mutagenecity and clastogenicity of heavy metals by physico-chemical factors.Environ. Res.37, 253–286 (1985).

    Article  PubMed  CAS  Google Scholar 

  • Failla M.L., Benedict C.D., Weinberg E.D.: Accumulation and storage of Zn2+ byCandida utillis.J. Gen. Microbiol.94, 23–36 (1976).

    PubMed  CAS  Google Scholar 

  • Failla M.L., Weinberg E.D.: Cyclic accumulation of zinc byCandida utilis during growth in batch culture.J. Gen. Microbiol.99, 85–97 (1977).

    PubMed  CAS  Google Scholar 

  • Gadd G.M., Griffith A.J.: Microorganisms and heavy metal toxicity.Microbial Ecol.4, 303–317 (1978).

    Article  CAS  Google Scholar 

  • Gadd G.M.: The uptake of heavy metals by fungi and yeasts: The chemistry and physiology of the process and applications for biotechnology, pp. 135–147 in H. Eccles, S. Hunt (Eds)Immobilization of Ions by Biosorption. Ellis Horwood, Chichester 1986.

    Google Scholar 

  • Gadd G.M.: Accumulation of metals by microorganisms and algae, pp. 402–433 in H.J. Rehm, G. Reed (Eds.):Biotechnology, Vol. 6b Verlag Chemie, Weinheim 1988.

    Google Scholar 

  • Grafl H.J., Schwantes H.O.: The effects of cadmium, zinc, lead and mercury on the growth and accumulating ability ofSaccharomyces cerevisiœ, Saccharomycopsis lipolytica, Candida tropicalis, andCandida utilis.Zbl. Bacteriol. Hyg. Ser. B177, 57–74 (1983).

    CAS  Google Scholar 

  • Itoh M., Yuasa M., Kobayashi T.: Adsorption of metal ions on yeast cells at varied cell concentrations.Plant Cell Physiol.16, 1167–1169 (1975).

    CAS  Google Scholar 

  • Minney S.F., Quirk A.V.: Growth and adaptation ofSaccharomyces cerevisiœ at different cadmium concentration.Microbios42, 37–44 (1985).

    PubMed  CAS  Google Scholar 

  • Mowll J.L., Gadd G.M.: Zinc uptake and toxicity in the yeastsSporobolomyces roseus andSaccharomyces cerevisiœ.J. Gen. Microbiol.129, 3421–3425 (1983).

    CAS  Google Scholar 

  • Mowll J.L., Gadd G.M.: Cadmium uptake byAureobasidium pullulans.J. Gen. Microbiol.130, 279–284 (1984).

    CAS  Google Scholar 

  • Norris P.R., Kelly D.P.: Accumulation of cadmium and cobalt bySaccharomyces cerevisiœ J. Gen. Microbiol.20, 299–308 (1977).

    Google Scholar 

  • Olson B.H., Panigrahi A.K.: Bacteria fungi, and blue-green algœ pp. 419–448 in E. Merian (Ed.):Metals and Their Compounds in the Environment. Verlag Chemie, Weinheim 1991.

    Google Scholar 

  • Perkin M.J., Ross I.S.: The specific uptake of managese in the yeastCandida utilis.J. Gen. Microbiol.132, 2155–2160 (1986).

    Google Scholar 

  • Somogyi M.: Notes on sugar determination.J. Biol. Chem.195, 19–23 (1952).

    CAS  Google Scholar 

  • Trevors J.T., Stratton G.W., Gadd G.M.: Cadmium transport, resistence, and toxicity in bacteria, algœ and fungi.Can. J. Microbiol.32, 447–464 (1986).

    Article  PubMed  CAS  Google Scholar 

  • Varian Publ. (Anonymous) 85-100009-00 (1979).

  • Voleský B.: Biosorption by fungal biomass. pp. 139–172 in B. Voleský (Ed.):Biosorption of Heavy Metals. CRC Press, Boca Raton 1990.

    Google Scholar 

  • Voleský B., Votruba J.:Modeling and Optimization of Fermentation Processes. Elsevier, Amsterdam 1992.

    Google Scholar 

  • Voleský B., May H., Holan Z.R.: Cadmium biosorption bySaccharomyces cerevisiœ.Biotechnol. Bioeng.41, 826–829 (1993).

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague 4

    P. Kujan, J. Votruba & V. Kameník

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  1. P. Kujan
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  2. J. Votruba
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  3. V. Kameník
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Kujan, P., Votruba, J. & Kameník, V. Substrate dependent bioaccumulation of cadmium by growing yeastCandida utilis . Folia Microbiol 40, 288–292 (1995). https://doi.org/10.1007/BF02814210

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  • DOI: https://doi.org/10.1007/BF02814210

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