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Microsatellite primer amplification by multiplexing: A first application toEucalyptus grandis

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Abstract

Multiplexing was used to increase the efficiency of microsatellite analysis inEucalyptus grandis. Primers were chosen based on the range of eucalyptus SSR alleles and their hybridization temperatures. Choice of PCR conditions proved to be the most important factor; concentrations of primers must be adjusted for use in combination. Results show that 6 primers can be analyzed in 2 PCR reactions, one containing 2 and the other containing 4 primers. Multiplexing promises to be a particularly efficient tool for microsatellite diversity analysis inEucalyptus grandis.

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Authors and Affiliations

  1. Cirad-forêt/Fofifa, Antananarivo 101, B.P. 745, Madagascar

    Gilles Chaix & Sylvie Razafiarivelo

  2. Cirad-emvt Campus International de Baillarguet TA, 30G 34398, Montpellier Cedex 5, France

    Isabelle Chantal & Jean Charles Maillard

  3. Cirad-forêt Campus International de Baillarguet TA, 10C 34398, Montpellier Cedex 5, France

    Mireille Poitel & Daniel Verhaegen

Authors
  1. Gilles Chaix
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  2. Isabelle Chantal
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  3. Mireille Poitel
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  4. Sylvie Razafiarivelo
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  5. Daniel Verhaegen
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  6. Jean Charles Maillard
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Correspondence to Gilles Chaix.

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Chaix, G., Chantal, I., Poitel, M. et al. Microsatellite primer amplification by multiplexing: A first application toEucalyptus grandis . Plant Mol Biol Rep 20, 67–68 (2002). https://doi.org/10.1007/BF02801934

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  • DOI: https://doi.org/10.1007/BF02801934

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