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Protocol for DNA extraction from potato tubers

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Abstract

A method to extract high-quality DNA from potato tubers was developed and tested on 3 wild potato species (Solanum raphanifolium, S. megistracrolobum, S. bukasovii) and on the tetraploid B3 bred population, (population number 393228, derived fromS. tuberosum subsp.tuberosum). The average yield of extracted DNA varied from 10–30 μg of DNA per gram of processed tissue. The DNA was pure and suitable for ligation-mediated polymerase chain reaction (LM-PCR) amplification, producing clear, distinctive, and reproducible banding patterns in polyacrylamide gels.

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  1. E. G. Wulff

    Present address: Department of Crop Protection, International Potato Center, Apartado 1558, Lima 12, Peru

Authors and Affiliations

  1. Department of Crop Protection, International Potato Center, Apartado 1558, Lima 12, Peru

    S. Torres & E. Gonzales Vigil

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  1. E. G. Wulff
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  2. S. Torres
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  3. E. Gonzales Vigil
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Correspondence to E. G. Wulff.

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Wulff, E.G., Torres, S. & Vigil, E.G. Protocol for DNA extraction from potato tubers. Plant Mol Biol Rep 20, 187 (2002). https://doi.org/10.1007/BF02799434

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  • DOI: https://doi.org/10.1007/BF02799434

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