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Antioxidant effect of vitamin E and glutathione on lipid peroxidation in boar semen plasma

  • Part I Free Radical Stress: Interactions with Trace Elements or Vitamins
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Abstract

The protective effect of vitamin E and reduced glutathione (GSH) against lipid peroxidation in boar semen plasma was studied. The lipid peroxidation, measured by the test for thiobarbituric acid reactive substances (TBARS), doubled in the presence of the lipid peroxidation Fe2+-sodium ascorbate-inducing system. The ascorbate-induced TBARS were inhibited by about 62% through the water-soluble vitamin E analog (TROLOX) and about 57% by GSH. In the in vivo experiments, 7 wk of oraldl-α-tocopherol acetate (1000 IU/d/animal) administration caused a significant fall in the level of the semen plasma TBARS, from 2.2±0.09 to 1.2±0.13 nmol MDA/mL. The semen plasma superoxide dismutase (SOD) and GSSG tended to increase with the time of vitamin E administration, but the increment did not reach a significant level by the seventh week. The vitamin E supplementation significantly increased the number of spermatozoa per 1 cm3 of ejaculate. The protective role of vitamin E and GSH with respect to boar semen against fatty acid peroxidation and a positive influence of vitamin E supplementation on semen quality have been evidenced.

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References

  1. H. A. Lardy and P. H. Phillips,J. Biol. Chem. 138, 195 (1941).

    CAS  Google Scholar 

  2. R. Jones and T. Mann,J. Reprod. Fert. 50, 255 (1977).

    Article  CAS  Google Scholar 

  3. R. Jones and T. Mann,J. Reprod. Fert. 50, 261 (1977).

    Article  CAS  Google Scholar 

  4. J. G. Alvarez and B. T. Storey,Biol. Reprod. 27, 1102 (1982).

    Article  PubMed  CAS  Google Scholar 

  5. B. Rao, J. C. Soufir, M. Martin and G. David,Gamete Res. 24, 127 (1989).

    Article  PubMed  CAS  Google Scholar 

  6. R. K. Dawra, O. P. Sharma, and H. P. S. Makkar,Int. J. Fertil. 28, 231 (1983).

    PubMed  CAS  Google Scholar 

  7. V. G. Pursel,Beltsville Symposia in Agriculture Research 3. Animal Reproduction. Allanheld, Osmun & Co., Montclair, NJ, pp. 145–157 (1979).

    Google Scholar 

  8. W. Diezel, S. Engel, N. Sonnichsen, and W. E. Horne.Andrologia 12, 167 (1980).

    Article  PubMed  CAS  Google Scholar 

  9. J. A. Buege and S. D. Aust,Methods Enzymol. 52, 302–310 (1978).

    Article  PubMed  CAS  Google Scholar 

  10. P. J. Hissin and R. Hilt,Anal. Biochem. 74, 214 (1976).

    Article  PubMed  CAS  Google Scholar 

  11. R. Kawaguchi, I. Sakurabayashi, T. Yamanaka, and T. Kawai,Japan. J. Clin. Chem. 12, 142 (1983).

    Google Scholar 

  12. E. F. Hartree,Anal. Biochem. 48, 422 (1972).

    Article  PubMed  CAS  Google Scholar 

  13. J. G. Alvarez and B. T. Storey,Biol. Reprod. 30, 833 (1984).

    Article  PubMed  CAS  Google Scholar 

  14. T. Laskowska-Klita and E. Szymańska,Animal Reprod. Sci.,20, 83 (1989).

    Article  CAS  Google Scholar 

  15. J. Strzezek, Z. Luberda and W. Demianowicz,Proceedings of the IX Congress of the Polish Soc. Vet. Sci., September 17–19, 1992, ART Olsztyn, Poland, p. 473 (in Polish) (1992).

  16. H. Wefers and H. Sies,Eur. J. Biochem. 174, 353 (1988).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Developmental and Experimental Endocrinology, Centre for Agrotechnology and Veterinary Sciences, Polish Academy of Sciences, ul. Grunwaldzka 250, Poznań, Poland

    E. Brzezińska-Ślebodzińska, A. B. Ślebodziński, B. Pietras & G. Wieczorek

Authors
  1. E. Brzezińska-Ślebodzińska
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  2. A. B. Ślebodziński
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  3. B. Pietras
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  4. G. Wieczorek
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Brzezińska-Ślebodzińska, E., Ślebodziński, A.B., Pietras, B. et al. Antioxidant effect of vitamin E and glutathione on lipid peroxidation in boar semen plasma. Biol Trace Elem Res 47, 69–74 (1995). https://doi.org/10.1007/BF02790102

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