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Selenium enhances glutathione peroxidase activity and prostacyclin release in cultured human endothelial cells

Concurrent effects on mRNA levels

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Abstract

Selenium (Se) is an essential component of glutathione peroxidase (GSH-Px), an enzyme that protects cells by reducing intracellular peroxides. Impaired Se status and GSH-Px activity seem associated with increased risk of atherosclerotic vascular diseases. This study reports the effects of Se supplementation on GSH-Px activity, on prostacyclin (PGI2) production, on 12-hydroxy-eicosatetraenoic acid (12-HETE) levels, and on GSH-Px mRNA expression in cultured human umbilical vein endothelial cells (HUVEC). Se-enriched HUVEC showed significant increase of both GSH-Px activity and thrombin-stimulated production of PGI2 in the presence of stable concentrations of 12-HETE. On the other hand, an inverse correlation between Se concentrations in culture media and GSH-Px mRNA levels in Northern blot analysis was shown; this suggests that a major degree of regulation for GSH-Px expression by Se is most likely exerted at the posttranscriptional level. These observations may help to explain the increased incidence of atherosclerosis described in Se-deficient individuals.

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Authors and Affiliations

  1. Cattedra di Ematologia, Valeggio s.M. Università di Verona, 37134, Verona, Italy

    M. M. Ricetti, R. Marrocchella, A. Rigo & G. Perona

  2. Laboratorio di Chimica Clinica C.O.C., Valeggio s.M. Università di Verona, 37134, Verona, Italy

    G. C. Guidi & G. Bellisola

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  1. M. M. Ricetti
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  2. G. C. Guidi
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  3. G. Bellisola
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  4. R. Marrocchella
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  5. A. Rigo
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  6. G. Perona
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Ricetti, M.M., Guidi, G.C., Bellisola, G. et al. Selenium enhances glutathione peroxidase activity and prostacyclin release in cultured human endothelial cells. Biol Trace Elem Res 46, 113–123 (1994). https://doi.org/10.1007/BF02790072

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  • DOI: https://doi.org/10.1007/BF02790072

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