Abstract
The present study was conducted to investigate the effect of zinc deficiency on fatty acid desaturation in rats fed two different types of dietary fat, a mixture of coconut oil and safflower oil (7∶1, w/w, “coconut oil diet”) or linseed oil (“linseed oil diet”). In order to ensure an adequate food intake, all rats were force-fed by gastric tube. Zinc deficiency caused statistical significant reducion of Δ9-desaturase activity in liver microsomes of rats fed coconut oil diet and tendencial reduction (p<0.15) in rats fed linseed oil diet compared with control rats fed diets with the same type of fat. In agreement with this effect, zinc deficiency in the rats fed both types of dietary fat increased the ratio between total saturated and total monounsaturated fatty in liver phospholipids and liver microsomes. Zinc deficient rats on the coconut oil diet had unchanged Δ6-desaturase activity with linoleic acid as substrate and lowered activity with α-linolenic acid as substrate. In contrast, zinc deficient rats on the linseed oil diet had increased Δ6-desaturase activity with linoleic acid as substrate and unchanged activity with α-linolenic acid. Because linoleic acid is the main substrate for Δ6-desaturase in the rats fed coconut oil diet, and α-linolenic acid is the main substrate in the rats fed linseed oil diet, it is concluded that in vivo Δ6-desaturation was not changed by zinc deficiency in the rats fed both types of dietary fat. Activity of Δ5-desaturase was also not changed by zinc deficiency in the rats fed both dietary fats. Levels of fatty acids in liver phospholipids and microsomes derived by Δ4-, Δ5-, and Δ6-desaturation were not consistently changed by zinc deficiency in the rats fed both types of dietary fat. Thus, the enzyme studies and also fatty acid composition data of liver phospholipids and microsomes indicate that zinc deficiency does not considerably disturb desaturation of linoleic and α-linolenic acid. Therefore, it is suggested that similarities between deficiencies of zinc and essential fatty acids described in literature are not due to disturbed desaturation of linoleic acid in zinc deficiency. The present study also indicates that zinc deficiency enhances incorporation of eicosapentaenoic acid into phosphatidylcholine of rats fed diets with large amounts ofn-3 polyunsaturated fatty acids.
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W. J. Bettger, P. G. Reeves, E. A. Moscatelli, G. Reynolds, and B. L. O'Dell,J. Nutr. 109, 480 (1979).
S. Ayala and R. R. Brenner,Acta Physiol. Latinoam,33, 191 (1983).
P. Chanmugan, C. Wheeler, and D. H. Hwang,J. Nutr.,114, 2073 (1984).
S. Clejan, M. Castro-Magana, P. J. Collipp, E. Jonas, and V. T. Maddaiah,Lipids 17, 129 (1982).
S. C. Cunnane, D. F. Horrobin, and M. S. Manku,Proc. Soc. Exp. Biol. Med. 177, 441 (1984).
S. C. Cunnane,Br. J. Nutr. 59, 273 (1988).
A. C. Fogerty, G. L. Ford, I. E. Dreosti, and I. J. Tinsley,Nutr. Rep. Int.,32, 1009 (1985).
H. P. Roth and M. Kirchgessner,J. Anim. Physiol. Anim. Nutr.,70, 236 (1993).
T. R. Kramer, M. Briske-Anderson, S. B. Johnson, and R. T. Holman,J. Nutr. 114, 1224 (1984).
K. Eder and M. Kirchgessner,Z. Ernährungswiss.32, 187 (1993).
S. Clejan, M. Castro-Magnana, and P. J. Collipp,Lipids 16, 454 (1981).
S. L. Tsai, M. C. Craig-Schmidt, J. D. Weete, and R. E. Keith,Fed. Am. Soc. Exp. Biol. 42, 823 (1983).
R. T. Holman, S. B. Johnson, O. Mercuri, H. J. Itarte, M. A. Rodrigo, and M. E. De Tomas,Am. J. Clin. Nutr. 34, 1534 (1981).
M. E. De Tomas, O. Mercuri, and A. Rodrigo,J. Nutr. 110, 595 (1980).
E. G. Hill and R. T. Holman,J. Nutr. 110, 1057 (1980).
T. Gerson,J. Nutr. 104, 701–709 (1974).
K. Eder and M. Kirchgessner,J. Nutr. 124, 1917 (1994).
A. Schülein, M. Kirchgessner, and H. P. Roth,J. Anim. Physiol. Anim. Nutr. 67, 157 (1992).
Y. H. Y. Park, C. J. Grandjean, D. L. Antonson, and J. A. Vanderhoof,J. Nutr. 116, 610 (1986).
P. R. Flanagan,J. Nutr. 114, 493 (1984).
T. R. Kramer, M. Briske-Anderson, S. B. Johnson, and R. T. Holman,Nutr. Res. 6, 1063 (1986).
K. Eder and M. Kirchgessner,Lipids 29, 839 (1994).
E. N. Christiansen, J. S. Lund, T. Rortveit, and A. C. Rustan,Biochim. Biophys. Acta 1082, 57–62 (1991).
M. L. Garg, E. Sebokova, A. B. R. Thomson, and T. M. Clandinin,Biochem. J. 249, 351 (1988).
R. R. Brenner, Factors influencing fatty acid chain elongation and desaturation, inThe Role of Fats in Human Nutrition, A. J. Vergroesen and M. Crawford, eds., Academic, London, pp. 45–79 (1989).
S. M. Actis Dato, A. Catala, and R. R. Brenner,Lipids 8, 1 (1973).
K. Smith, R. J. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, M. Goeke, B. J. Olson, and D. C. Klenk,Anal. Biochem. 150, 76 (1985).
W. R. Morrison and L. M. Smith,J. Lipid Res. 5, 600 (1964).
M. Narce, J. Gresti, and J. Bezard,J. Chromatogr. 448, 249 (1988).
A. Hara and N. S. Radin,Anal. Biochem. 90, 420 (1978).
K. Eder, A. M. Reichlmayr-Lais, and M. Kirchgessner,J. Chromatogr. 598, 33 (1992).
K. Eder, A. M. Reichlmayr-Lais, and M. Kirchgessner,J. Chromatogr. 588, 265 (1991).
R. R. Brenner and R. O. Peluffo,J. Biol. Chem. 241, 5213 (1966).
N. Nudo, Y. Nakagawa, and K. Waku,Biol. Trace Elem. Res.,24, 49 (1990).
R. Christon, Y. Fernandez, C. Cambon-Gros, A. Periquet, P. Deltour, C. L. Leger, and S. Mitjavila,J. Nutr.,118, 1311 (1988).
K. M. Hargreaves, D. J. Pehowich, and M. T. Clandinin,J. Nutr.,119, 344 (1989).
W. E. Cornatzer, J. A. Haning, J. C. Wallwork, and H. H. Sandstead,Biol. Trace Elem. Res. 6, 393 (1984).
W. J. Bettger and B. L. O'Dell,Life Sci.,28, 1425 (1981).
C. D. Stubbs and A. D. Smith,Biochim. Biophys. Acta,779, 89 (1984).
J. F. Mead,J. Lipid Res.,25, 1517 (1984).
B. L. O'Dell, J. D. Browning, and P. G. Reeves,J. Nutr.,117, 1883 (1987).
P. Chanmugan, C. Wheeler, and D. H. Hwang,J. Nutr. 114, 2066 (1984).
D. H. Hwang, P. Chanmugan, and C. Wheeler,J. Nutr.,114, 398 (1984).
M. K. Song and N. F. Adham,Am. J. Clin. Nutr. 41, 1201 (1985).
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Eder, K., Kirchgessner, M. Activities of liver microsomal fatty acid desaturases in zinc-deficient rats force-fed diets with a coconut oil/safflower oil mixture of linseed oil. Biol Trace Elem Res 48, 215–229 (1995). https://doi.org/10.1007/BF02789404
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DOI: https://doi.org/10.1007/BF02789404