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Reporter enzymes for the study of promoter activity

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Abstract

This article describes the use of three reporter enzymes used to study promoter activity in transgenic animals. Chloramphenicol acetyl transferase may be assayed by a nonchromatographic method that is rapid and sensitive. β-Galactosidase is measured by a photometric assay and luciferase is assayed by measuring the emission of light using a luminometer. The relative merits of each enzyme is discussed. Ths use of reporter enzymes provides a rapid and sensitive method for analysis of transgene expression.

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  1. 9 Velindre Place, Whitchurch, Cardiff, CF4 2AN, Wales, UK

    Karen Pardy

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  1. Karen Pardy
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Pardy, K. Reporter enzymes for the study of promoter activity. Mol Biotechnol 2, 23–27 (1994). https://doi.org/10.1007/BF02789287

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  • DOI: https://doi.org/10.1007/BF02789287

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