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Determination of reference ranges for elements in human scalp hair

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Abstract

Expected values, reference ranges, or reference limits are necessary to enable clinicians to apply analytical chemical data in the delivery of health care. Determination of references ranges is not straightforward in terms of either selecting a reference population or performing statistical analysis. In light of logistical, scientific, and economic obstacles, it is understandable that clinical laboratories often combine approaches in developing health associated reference values. A laboratory may choose to:

  1. 1.

    Validate either reference ranges of other laboratories or published data from clinical research or both, through comparison of patients test data.

  2. 2.

    Base the laboratory’s reference values on statistical analysis of results from specimens assayed by the clinical reference laboratory itself.

  3. 3.

    Adopt standards or recommendations of regulatory agencies and governmental bodies.

  4. 4.

    Initiate population studies to validate transferred reference ranges or to determine them anew.

Effects of external contamination and anecdotal information from clinicians may be considered.

The clinical utility of hair analysis is well accepted for some elements. For others, it remains in the realm of clinical investigation. This article elucidates an approach for establishment of reference ranges for elements in human scalp hair. Observed levels of analytes from hair specimens from both our laboratory’s total patient population and from a physician-defined healthy American population have been evaluated. Examination of levels of elements often associated with toxicity serves to exemplify the process of determining reference ranges in hair. In addition the approach serves as a model for setting reference ranges for analytes in a variety of matrices.

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References

  1. How to define and determine reference intervals in the clinical laboratory; approved guideline, NCCLS (National Committee for Clinical Laboratory Standards)15(4), 1–4, June 1995.

  2. M. K. Sandford and G. E. Kissling, Multivariate analyses of elemental hair concentrations from a medieval Nubian population,Am. J. Phys. Anthropol. 95(1), 41–52 (1994).

    Article  PubMed  CAS  Google Scholar 

  3. C. Chai, W. Feng, Q. Qian, M. Guan, X. Li, Y. Lu, et al., Total and methyl mercury levels in human scalp hairs of typical populations in China by NAA, GC(EC), and other techniques,Biol. Trace Element Res. 43-45, 423–433 (1994).

    CAS  Google Scholar 

  4. H. Mussalo-Rauhamaa, M. Kantola, K. Seppanen, L. Soininen, and M. Koivusalo, Trends in the concentrations of mercury, copper, zinc and selenium in inhabitants of north-eastern Finnish Lapland in 1982–1991. A pilot study,Arctic Med. Res. 55(2), 83–91 (1996).

    PubMed  CAS  Google Scholar 

  5. R. K. Dogra, R. C. Murthy, A. L. Srivastava, J. S. Gaur, L. J. Shukla, and B. M. Varmani, Cattle mortality in the Thane district, India: a study of cause/effect relationships,Arch. Environ. Contam. Toxicol. 30(2), 292–297 (1996).

    PubMed  CAS  Google Scholar 

  6. V. Bencko, T. Geist, D. Arbetova, D. M. Dharmadikari, and E. Svandova,. Biological monitoring of environmental pollution and human exposure to some trace elements,J. Hygiene, Epidemiol., Microbiol. Immunol. 30, 1–10 (1986).

    CAS  Google Scholar 

  7. S. C. Foo, N. Y. Khoo, A. Heng, L. H. Chua, S. E. Chia, C. N. Ong, et al., Metals in hair as biological indices for exposure,Int. Arch. Occup. Environ. Health 65, S83-S86 (1993).

    Article  PubMed  CAS  Google Scholar 

  8. Y. S. Ryabukhin, Nuclear-based methods for the analysis of trace element pollutants in human hair,J. Radioanal. Chem. 60(1), 7–30 (1980).

    Article  CAS  Google Scholar 

  9. A. Taylor, Usefulness of measurements of trace elements in hair,Ann. Clin. Biochem. 23, 364–378 (1986).

    PubMed  CAS  Google Scholar 

  10. R. F. Puchyr, D. A. Bass, R. Gajewski, M. Calvin, W. Marquardt, M. E. Druyan, et al, Preparation of hair for elemental measurement by ICP-MS, this issue,Biol. Trace Element Res., in press.

  11. Y. S. Ryabukin, Activation analysis of hair as an indicator of contamination of man by environmental trace element pollutants, IAEA report, 1AEA/RL/50, Vienna (1978).

  12. S. J. Haswell and D. Barclay, On-line microwave digestion of slurry samples with direct flame atomic absorption spectrometric elemental detection,Analyst 117, 117–120 (1992).

    Article  CAS  Google Scholar 

  13. E. S. Beary, P. J. Paulsen, L. B. Jassie, and J. D. Fasset, Determination of environmental lead using continuous-flow microwave digestion isotope dilution inductively coupled plasma mass spectrometry,Anal. Chem. 69, 758–766 (1997).

    Article  CAS  Google Scholar 

  14. M. E. Druyan, R. F. Puchyr, A. W. Fisher, E. Harmon, K. E. Urek, D. W. Quig, et al., Interpretation of elemental analyses for clinical application, paper presented at Great Lakes Regional Meeting, American Chemical Society, May 28–30 (1997).

  15. R. A. Buckley and I. E. Dreosti, Radioisotopic studies concerning the efficacy of standard washing procedures for the cleansing of hair before zinc analysis,Am. J. Clin. Nutr. 40, 840–846 (1984).

    PubMed  CAS  Google Scholar 

  16. S. Salmela, E. Vuori, and J. O. Kilpio, The effect of washing procedures on trace element content of human hair,Anal. Chim. Acta. 125, 1131–1137 (1981).

    Article  Google Scholar 

  17. J. M. McKenzie, Alteration of the zinc and copper concentration of hair,Am J. Clin. Nutr. 31, 470–76 (1978).

    PubMed  CAS  Google Scholar 

  18. D. C. Hilderbrand and D. H. White, Trace element analysis in hair: an evaluation,Clin. Chem. 20, 148–151 (1974).

    PubMed  CAS  Google Scholar 

  19. G. S. Assarian and D. Aberleas, Effect of washing procedures on trace element content of hair,Clin. Chan. 23, 1771, 1772 (1972).

    Google Scholar 

  20. W. W. Harrison, J. P. Yarachek, and C. A. Benson, The determination of trace elements in human hair by atomic absorption spectroscopy,Clin. Chim. Acta 23, 83–91 (1969).

    Article  PubMed  CAS  Google Scholar 

  21. R. Ekins and P. Edwards, Point on the meaning of “sensitivity,”Clin. Chem. 43(10), 1824–1827 (1997).

    PubMed  CAS  Google Scholar 

  22. D. Krieger, S. Krieger, O. Jansen, P. Gass, L. Theilmann, and H. Lichtnecker, Manganese and chronic hepatic encephalopathy,The Lancet 346, 270–274 (1995).

    Article  CAS  Google Scholar 

  23. International Programme on Chemical Safety: Environmental Health Criteria 118 Inorganic Mercury, World Health Organization, Geneva, pp. 60, 61 (1991).

  24. J. T. Salonen, K. Seppänen, K. Nyssönen, H. Korpela, J. Kauhanen, M. Kantola, et al, Intake of mercury from fish, lipid peroxidation, and the risk of myocardial infarction and coronary, cardiovascular, and any death in eastern Finnish men,Circulation 91(3), 646–655 (1995).

    Google Scholar 

  25. Inorganic Mercury, Environmental Health Criteria 118, World Health Organization, Geneva (1991).

  26. G.-G. Elinder, L. Gerhardsson, and G. Oberdoerster, Biological monitoring of metals, in:Biological Monitoring of Metals, T. W. Clarkson, L. Friberg, G. F. Nordberg, and P. Sager, eds., Plenum, London, pp. 1–71 (1988).

    Google Scholar 

  27. SPSS Base 7.5,Applications Guide, SPSS Inc., Chicago, IL, pp. 27, 28 (1997).

    Google Scholar 

  28. W. W. Daniel,Biostatistics: A Foundation for Analysis in the Health Sciences, 3rd ed.,Wiley Series in Probability and Mathematical Statistics-Applied, Wiley, New York (1983).

    Google Scholar 

  29. D. Das, A. Chatterjee, B. K. Mandai, G. Samanta, D. Chakraborti, and B. Chanda, Arsenic in ground water in six districts of West Bengal, India; the biggest arsenic calamity in the world. Part 2. Arsenic concentration in drinking water, hair, nails, urine, skin-scale and liver tissue of the affected people,Analyst 12(3), 917–924 (1995).

    Article  Google Scholar 

  30. R. Heaven, M. Duncan, and S. Vukelja, S., Arsenic intoxication presenting with macrocytosis and peripheral neuropathy, without anemia,Acta Haematol. 92(3), 142, 143 (1994).

    Article  PubMed  CAS  Google Scholar 

  31. M. Marlow and C. Moon, Correlations of metal-metal interactions as measured in hair on childhood intelligence,J. Adv. Med. 1(4), 195–203 (1988).

    Google Scholar 

  32. J. J. Powell, S. M. Greenfield, R. P. Thompson, J. A. Cargnello, M. D. Kindall, J. P. Landsberg, et al., Assessment of Toxic metal exposure following the Camelford water pollution incident; evidence of acute mobilization of lead into drinking water,Analyst 120(3), 793–798 (1995).

    Article  PubMed  CAS  Google Scholar 

  33. B. Minder, E. A. Das-Smaal, E. F. Brand, and J. F. Orlebeke, Exposure to lead and specific attentional problems in school children,J. Learning Disabilities 27(6), 393–399 (1994).

    CAS  Google Scholar 

  34. F. Watt, J. Landsberg, J. J. Powell, R. J. Ede, R. P. Thompson, and J. A. Cargnello, Analysis of copper and lead in hair using the nuclear microscope; results from normal subjects and patients with Wilson’s disease and lead poisoning,Analyst 120(3), 789–791 (1995).

    Article  PubMed  CAS  Google Scholar 

  35. O. Malm, F. J. Branches, H. Akagi, M. B. Castro, W. C. Pfeiffer, M. Harada, et al., Mercury and methylmercury in fish and human hair from the Tapajos River basin, Brazil,Sci. Total Environ. 175(20), 141–150 (1995).

    PubMed  CAS  Google Scholar 

  36. L. Holsbeek, H. K. Das, and C. R. Joiris, Mercury in human hair and relation to fish consumption,Bangladesh. Sci. Total Environ. 186(3), 181–188 (1996).

    Article  CAS  Google Scholar 

  37. T. Abe, R. Ohtsuka., T. Hongo, T. Suzuki, C. Tohyama, A. Nakano, et al., High hair and urinary mercury levels of fish eaters in the nonpolluted environment of Papua New Guinea,Arch. Environ. Health 50(5), 367–373 (1995).

    Article  PubMed  CAS  Google Scholar 

  38. W. Ashraf, M. Jaffar, D. Mohammed, and J. Iqbal, Utilization of scalp hair for evaluating epilepsy in male and female groups of the Pakistan population,Sci. Total Environ. 164(1), 69–73 (1995).

    Article  PubMed  CAS  Google Scholar 

  39. E. Contiero and M. Folin, Trace elements nutritional status. Use of hair as a diagnostic tool,Biol. Trace Element Res. 40(2), 151–160 (1994).

    CAS  Google Scholar 

  40. S. Loranger and J. Zayed, Environmental and occupational exposure to manganese: a multimedia assessment,Int. Arch. Occup. Environ. Health 67(2), 101–110 (1995).

    Article  PubMed  CAS  Google Scholar 

  41. J. Chatterjee, B. B. Mukherjee, K. De, A. K. Das, and S.K. Basu, Biological trace metal levels of X-ray technicians’ blood and hair,Trace Elem. Res. 46(3), 211–227 (1994).

    CAS  Google Scholar 

  42. National Research Council,Recommended Dietary Allowances, 10th ed. National Academy Press, Washington, DC (1989).

    Google Scholar 

  43. L. W. Chang, Toxico-neurology and neuropathology induced by metals, inToxicology of Metals, L. W. Chang, ed., CRC, Boca Raton, New York, pp. 511–535 (1996).

    Google Scholar 

  44. E. J. Massaro, The developmental cytotoxicity of mercurials, inToxicology of Metals, L. W. Chang, ed., CRC, Boca Raton, New York, pp. 1047–1081 (1996).

    Google Scholar 

  45. D. W. Jenkins,Biological Monitoring of Toxic Trace Metals, vol. 1.,Biological Monitoring and Surveillance, EPA Document 600—80-089, Las Vegas, NE (1980).

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Authors and Affiliations

  1. Doctor’s Data, Inc., 170 W. Roosevelt Road, 60185, West Chicago, IL

    Mary Ellen Druyan, Dean Bass, Richard Puchyr, Karen Urek, David Quig, Emmett Harmon & William Marquardt

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  1. Mary Ellen Druyan
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Druyan, M.E., Bass, D., Puchyr, R. et al. Determination of reference ranges for elements in human scalp hair. Biol Trace Elem Res 62, 183–197 (1998). https://doi.org/10.1007/BF02783970

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  • DOI: https://doi.org/10.1007/BF02783970

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