Abstract
The conditions and acid strength of the phenol/sulfuric acid assay were investigated to improve agreement between absorbances obtained from different sugars. It was found that by increasing acid strength and by cooling the tubes in water after a short reaction time, the values obtained for several sugars, including fructose and xylose, agreed, on an equimolar value, with that for glucose.
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Taylor, K.A.C.C. A modification of the phenol/sulfuric acid assay for total carbohydrates giving more comparable absorbances. Appl Biochem Biotechnol 53, 207–214 (1995). https://doi.org/10.1007/BF02783496
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DOI: https://doi.org/10.1007/BF02783496