Summary
Human Placental Anemia Inducing Factor (PAIF) was purified from crude placental preparation called P-62, which had common biological and immunological natures with the same preparation from gastric juice, gastric tissues, serum and urine of patients with malignant neoplasma, especially gastric cancer1-4). The obtained PAIF was homogeneous on polyacrylamide gel electrophoresis and gave a specific band in the region of γ-A and/or γ-M globulin on immunoelectrophoresis.
The purified PAIF induced anemia in rabbits by intravenous administration at a dose of 2.7 Μg/Kg of body weight. No cross antigenicity of PAIF was observed with α-Fetoprotein, HBs antigen and CEA.
Serum AIF of patients was measured using PAIF as a standard by radioimmunoassay. AIF levels in sera of patients with malignant neoplasma were significant high compared with those of control groups.
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References
Oh-Uti K, Suzuki K, Yamaguchi I: Toxic substances in cancer V. Anemia-inducing substances in the tissues of gastric carcinoma. Tohoku J exp Med 67: 115–121, 1958
Oh-Uti K, Shindo K, Mikami T, Tanimura T: Extraction of anemia-inducing substances from the human placenta and its chemical nature. Tohoku J exp Med 95: 369–377, 1968
Oh-Uti K, Shindo K, Numata T: Immunological in vestigation on the common antigenicity between anemia-inducing substances in cancerous gastric juice and in human placenta. Tohoku J exp Med 96: 151–159, 1968
Oh-Uti K, Shindo K, Tamura Y, Obara K, Numata T, Yamagata N, Kitajima S: The influence of anemiainducing organ extracts on osmotic fragility of the rabbit red cells in vitro. Tohoku J exp Med 95: 395–402, 1968
Zachrius RM, et al: Glycoprotein staining following electrophoresis on acrylamide gels. Anal Biochem 30: 148–152, 1969
McKelvy JF, Lee YC: Microheterogeneity of the carbohydrate group of Aspergillus oryzae α-amylase. Arch Biochem Biophys 132: 99–110, 1969
Crestfield AM, Moore S, Stein WH: The preparation and enzymatic hydrolysis of reduced and S-carboxy-methylated proteins. J Biol Chem 238: 622–627, 1963
Goodwin TW, Morton RA: The spectrometric determination of tyrosine and tryptophan in proteins. Biochem J 40: 628–632, 1946
Hunter WM, Greenwood FC: Preparation of iodine-131 labeled human growth hormone of high specific activity. Nature (Lond) 194: 495–498, 1962
Ariumi M: Malignant tumors and anemia IX. Immunological studies on a mucoprotein obtained from cancerous serum (in Jpn). Hirosaki Igaku 21: 484–516, 1970
Bergstrand CG, Czar B: Demonstration of a new protein fraction in serum from the human fetus. Scand J clin Lab Invest 8: 174, 1956
Bohn H: Nachweise und Charakterisierung von loslichen Antigenen in der menschlichen Placenta. Arch Gynaekol 212: 165–175, 1972
Oh-Uti K, Inoue S, Minato S: Purification of an anemia-inducing factor from human placenta and its application to diagnosis of malignant neoplasms. Cancer Res 40: 1686–1690, 1980
Roger GT, Searle F, Bagshawe KD: Heterogeneity of carcinoembryonic antigen and its fraction by con A affinity chromatography. Nature (Lond) 251: 519–520, 1974
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Inoue, S., Itoh, T., Torii, Y. et al. Purification of placental anemia inducing factor (PAIF) and its clinical application by radioimmunoassay. Gastroenterol Jpn 17, 93–97 (1982). https://doi.org/10.1007/BF02774546
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DOI: https://doi.org/10.1007/BF02774546