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A simple and rapid method for nuclear run-on transcription assays in plants

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Abstract

Nuclear run-on transcription assays allow researchers to determine if a gene is regulated at the transcriptional level. Existing methods to perform this procedure involve the application of a large variety of biochemical reagents, numerous steps, and time-consuming procedures. Here we report a method to perform this assay on plant tissue that involves a minimal number of reagents and steps, is time-efficient, and produces clean definitive results within 24 h.

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Authors and Affiliations

  1. Department of Plant and Microbial Biology, University of California, 94720-3102, Berkeley, USA

    Ling Meng & Peggy G. Lemaux

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  1. Ling Meng
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  2. Peggy G. Lemaux
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Meng, L., Lemaux, P.G. A simple and rapid method for nuclear run-on transcription assays in plants. Plant Mol Biol Rep 21, 65–71 (2003). https://doi.org/10.1007/BF02773398

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  • DOI: https://doi.org/10.1007/BF02773398

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