Skip to main content
SpringerLink
Log in

A novel method for in vitro culture of plants: Cultivation of barley in a floating hydroponic system

  • Protocols
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

We describe a system for hydroponically growing plants that is based on using a polyethylene (PE) granulate as the floating body. The procedure can be performed in all suitable vessels (e.g., bowls, basins, glasses) and is independent of the culture area. Barley plants (Hordeum vulgare) grown in this hydroponic system had no difference in the uptake of mercuric and cadmium ions when compared with plants grown on a defined agar medium. The simple and inexpensive method can be used for the isolation of leaf tissue and large amounts of root tissue. In addition, numerous experiments can be run in parallel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

PE:

polyethylene

References

  • Arteca RN and Arteca JM (2000) A novel method for growingArabidopsis thaliana plants hydroponically. Physiol Plant 108: 188–193.

    Article  CAS  Google Scholar 

  • Feng J, Zheng SJ, Li XF, Takeda K, and Matsumoto H (1997) A rapid hydroponic screening for aluminium tolerance in barley. Plant Soil 191: 133–137.

    Article  Google Scholar 

  • Gibeaut DM, Hulett J, Cramer GR, and Seemann JR (1997) Maximal biomass ofArabidopsis thaliana using a simple, low-maintenance hydroponic method and favourable environmental conditions. Plant Physiol 115: 317–319.

    Article  PubMed  CAS  Google Scholar 

  • Heidenreich B (1999) Transkriptionsänderungen vonArabidopsis thaliana bei Ozon-, UV-B und HgCl2-Behandlung. PhD thesis, LMU München.

  • Huttner D and Bar-Zvi D (2003) An improved, simple, hydroponic method for growingArabidopsis thaliana. Plant Mol Biol Rep 21: 59–63.

    Article  Google Scholar 

  • Schramel P and Wendler I (1998) Capabilities of double focussing magnetic sector-ICP-MS for the determination of trace elements in body fluids (blood, blood serum, urine) et the example of control materials. Fresenius J Anal Chem 361: 487–491.

    Article  CAS  Google Scholar 

  • Thiel S, Döhring T, Köfferlein M, Kosak A, Martin P, and Seidlitz HK (1996) A phytotron for plant stress research: How far can artificial lighting compare to natural sunlight? J Plant Physiol 148: 456–463.

    Article  CAS  Google Scholar 

  • Tocquin P, Corbesier L, Havelange A, Pieltain A, Kurtem E, Bernier G, and Périlleux C (2003) A novel high efficiency, low maintenance, hydroponic system for synchronous growth and flowering ofArabidopsis thaliana. BMC Plant Biol 3: 2.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biochemical Plant Pathology, GSF-National Research Centre for Environment and Health, D-85764, Neuherberg, Germany

    Florian Battke & Dieter Ernst

  2. Institute of Ecological Chemistry, GSF-National Research Centre for Environment and Health, D-85764, Neuherberg, Germany

    Peter Schramel

  3. TUM-Lehrstuhl für Pflanzenbau und Pflanzenzüchtung, Alte Akademie 12, D-85350, Freising-Weihenstephan, Germany

    Florian Battke

Authors
  1. Florian Battke
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter Schramel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dieter Ernst
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Battke, F., Schramel, P. & Ernst, D. A novel method for in vitro culture of plants: Cultivation of barley in a floating hydroponic system. Plant Mol Biol Rep 21, 405–409 (2003). https://doi.org/10.1007/BF02772589

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02772589

Key words

Search

Navigation