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RNA isolation from high-phenolic tea leaves and apical buds

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Abstract

We describe here a modified procedure based on guanidine hydrochloride to isolate RNA from apical buds of tea containing high levels of polyphenols. The major change was to increase β-mercaptoethanol from 50 to 200 mM in the extraction buffer. This produced phenolic-free and undegraded RNA. The procedure was extended to fully expanded leaves of tea and apical buds of other plant species. The total RNA could be used for mRNA isolation, reverse transcription, and differential display of mRNA. A clean-up protocol to remove chlorophylls that coprecipitate with the RNA also is presented.

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Authors and Affiliations

  1. Biotechnology Division, Institute of Himalyan Bioresource Technology, P.O. BOX #6, 176 061, Palampur, (HP), India

    Lakhvir Lal, Rashmita Sahoo, Rajesh Kumar Gupta & Sanjay Kumar

  2. Division of Agricultural Biotechnology, Himachal Pradesh Krishi Vishvavidyalya, 176 062, Palampur

    Priti Sharma

Authors
  1. Lakhvir Lal
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  2. Rashmita Sahoo
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  3. Rajesh Kumar Gupta
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  4. Priti Sharma
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  5. Sanjay Kumar
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Corresponding author

Correspondence to Sanjay Kumar.

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Lal, L., Sahoo, R., Gupta, R.K. et al. RNA isolation from high-phenolic tea leaves and apical buds. Plant Mol Biol Rep 19, 181 (2001). https://doi.org/10.1007/BF02772161

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  • DOI: https://doi.org/10.1007/BF02772161

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