Abstract
Three- or 4-year-old Japanese black pine seedlings were exposed to simulated acid rain (SAR) at pH 3 for two months, then inoculated with a virulent isolate (S 10) of pinewood nematodes. The experiments were repeated three times in 1996–1998. The exposure to SAR killed no seedlings, and retarded the development of disease symptoms in the seedlings inoculated with nematodes. In the experiments in 1996 and 1997, however, cessation of resin exudation, and primary decrease in the xylem conductivity occurred earlier in the seedlings exposed to SAR than in those exposed to tap water as a control. These results imply that acid rain at pH 3 influenced an increase in some resistance of Japanese black pine seedlings to pinewood nematode, and that this improved resistance could potentially overcome damage caused by acid rain. The exposure to SAR did not have any significant effect on the water relations of the seedlings itself, suggesting that retardation of the symptom development after the exposure to SAR could not be attributed to the improvement in the water relations.
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Asai, E. and Futai, K. (2001) The effects of long-term exposure to simulated acid rain on the development of pine wilt disease caused byBursaphelenchus xylophilus. For. Pathol. 31: 241–253.
Asai, E., Hata, K., and Futai, K. (1998) Effect of simulated acid rain on the occurrence ofLophodermium on Japanese black pine needles. Mycol. Res. 102: 1316–1318.
Bolla, R.I. and Fitzsimmons, K. (1988) Effect of simulated acid rain inBursaphelenchus xylophilus infection of pine seedlings. J. Nematol. 20: 590–598.
Eamus, D., Leith, I., and Fowler, D. (1989) Water relations of red spruce seedlings treated with acid mist. Tree Physiol. 5: 387–397.
Fukuda, K. (1997) Physiological process of the symptom development and resistance mechanism in pine wilt disease. J. For. Res. 2: 171–181.
Fukuda, K., Hogetsu, T., and Suzuki, K. (1992) Photosynthesis and water status of pine-wood nematode-infected pine seedlings. J. Jpn. For. Soc. 74: 1–8.
Futai, K. (1980) Population dynamics ofBursaphelenchus lignicolus (Nematoda: Aphelencoididae) andB. mucronatus in pine seedlings. Appl. Entomol. Zool. 15: 458–464.
Futai, K. and Furuno, T. (1979) The variety of resistances among pine species to pine wood nematode,Bursaphelenchus lignicolus. Bull. Kyoto Univ. For. 51: 23–36.
Futai, K. and Harashima, S. (1990) Effect of simulated acid mist on pine wilt disease. J. Jpn. For. Soc. 72: 520–523.
Futai, K. and Sutherland, J.R. (1989) Pathogenicity and attraction to host extracts of Canadian pinewood nematodes: Studies with Scots pine, western larch, and black spruce seedlings. Can. J. For. Res. 19: 1256–1261.
Huttunen, S. (1984) Interactions of disease and other stress factors with atmospheric pollution.In Air pollution and plant life. Treshow, M. (ed.), 486pp, John Wiley & Sons, Chichester, 321–356.
Igawa, M., Kameda, H., Maruyama, F., Okochi, H., and Otsuka, I. (1997) Effect of simulated acid fog on needles of fir seedlings. Environ. Exp. Bot. 38: 155–163.
Ikeda, T. (1996) Responses of water-stressedPinus thunbergii to inoculation with avirulent pine wood nematode (Bursaphelenchus xylophilus): Water relations and xylem histology. J. For. Res. 1: 223–226.
Ikeda, T. and Kiyohara, T. (1995) Water relations, xylem embolism and histological features ofPinus thunbergii inoculated with virulent or avirulent pine wood nematode,Bursaphelenchus xylophilus. J. Exp. Bot. 46: 441–449.
Ikeda, T. and Suzaki, T. (1984) Influence of pine-wood nematodes on hydraulic conductivity and water status inPinus thunbergii. J. Jpn. For. Soc. 66: 412–420.
Ikeda, T., Kiyohara, T., and Kusunoki, M. (1990) Change in water status ofPinus thunbergii Parl. inoculated with species ofBursaphelenchus. J. Nematol. 22: 132–135.
Johnson, J.D., Chappelka, A.H., Hain, F.P., and Heagle, A.S. (1995) Interactive effects of air pollutants with abiotic and biotic factors on southern pine forests.In Impact of air pollutants on southern pine forests. Fox, S. and Mickler, R.A. (eds.), 513pp, Springer-Verlag, New York, 281–312.
Kaneko, T., Nakai, I., and Ando, M. (1993) Measurement of acid rain using commercially sold acid rain collector: Changes of pH at the beginning of a rain. Trans. Meet. Kansai Branch Jpn. For. Soc. 2: 57–58. (in Japanese)
Kishi, Y. (1995) The pinewood nematode and Japanese pine sawyer. 302pp, Thomas Company, Tokyo.
Kiyohara, T. and Suzuki, K. (1978) Nematode population growth and disease development in the pine wilting disease. Eur. J. For. Pathol. 8: 285–292.
Kohno, Y., Matsumura, H., and Kobayashi, T. (1994) Effect of simulated acid rain on the development of leaf injury in tree seedlings. J. Jpn. Atmos. Environ. 29: 206–219. (in Japanese with English summary)
Lee, W.S., Chevone, B.I., and Seiler, J.R. (1990) Growth and gas exchange of loblolly pine seedlings as influenced by drought and air pollutants. Water Air Soil Pollut. 51: 105–116.
Mamiya, Y. (1985) Initial pathological changes and disease development in pine trees induced by the pine wood nematode,Bursaphelenchus xylophilus. Ann. Phytopathol. Soc. Jpn. 51: 546–555.
Mengel, K., Hogrebe, A.M. R., and Esch, A. (1989) Effect of acidic fog on needle surface and water relations ofPicea abies. Physiol. Plant. 75: 201–207.
Nakano, M., Miyazaki, T., Shiozawa, S., and Nishimura, T. (1995) Physical and environmental analysis of soils. 236pp, University of Tokyo Press, Tokyo. (in Japanese)
Ohkita, T. (1996) Acid rain (new edition)—Compound effects and the influences on ecosystems—. 309pp, Hakuyuu-sha, Tokyo. (in Japanese)
Saegusa, M. (1994) Chemistry of aluminum in acidic soil.In Low pH soil and plants. Inoue, K. and Mori, S. (eds.), 236pp, Hakuyuusha, Tokyo, 7–42. (in Japanese)
Suzuki, K. (1984) General effect of water stress on the development of pine wilting disease caused byBursaphelenchus xylophilus. Bull. Forest For. Prod. Res. Inst. 325: 97–126. (in Japanese with English summary)
Taketani, A., Okuda, M., and Hosoda, R. (1975) The meteorological analysis on the epidemic mortality of pine trees, with special reference to the effective accumulated temperature. J. Jpn. For. Soc. 57: 169–175. (in Japanese with English abstract)
Taketsune, A. (1992) Effects of acid rain and acid fog on pine wilt disease (I). Detection of pine wood nematode (Bursaphelenchus xylophilus Steineret Bührer) from dead trees in the foggy district and tests with simulated acid water sprayed to seedlings inoculated with this nematode. Bull. Hiroshima Pref. For. Exp. Sta. 26: 23–30. (in Japanese with English summary)
Worrall, J.J. (1994) Relationships of acid deposition and sulfur dioxide with forest disease.In Effects of acid rain on forest processes. Godbold, D.L. and Hüttermann, A. (eds.) 419pp, Wiley-Liss, New York, 163–182.
Yamada, T. and Ito, S. (1993) Histological observations on the responses of pine species,Pinus strobus andP. taeda, resistant toBursaphelenchus xylophilus infection. Ann. Phytopathol. Soc. Jpn. 59: 659–665.
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This work was supported in part by a Grants-in-Aid for Scientific Research from the Japan Forest Technical Association (JAFTA).
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Asai, Ei., Futai, K. Retardation of pine wilt disease symptom development in Japanese black pine seedlings exposed to simulated acid rain and inoculated withBursaphelenchus xylophilus . J For Res 6, 297–302 (2001). https://doi.org/10.1007/BF02762471
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DOI: https://doi.org/10.1007/BF02762471