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Phage-display libraries of murine and human antibody Fab fragments

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Abstract

We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

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Authors and Affiliations

  1. Department of Biology, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100, Copenhagen, Denmark

    Jan Engberg, Peter Sejer Andersen, Lene K. Johansen & Bjarne Albrechtsen

  2. Blodbanken KI2031, Department of Clinical Immunology, University Hospital, Blegdamsvej 9, DK-2100, Copenhagen, Denmark

    Leif Kofoed Nielsen & Morten Dziegiel

Authors
  1. Jan Engberg
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  2. Peter Sejer Andersen
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  3. Leif Kofoed Nielsen
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  4. Morten Dziegiel
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  5. Lene K. Johansen
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  6. Bjarne Albrechtsen
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Corresponding author

Correspondence to Jan Engberg.

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Engberg, J., Andersen, P.S., Nielsen, L.K. et al. Phage-display libraries of murine and human antibody Fab fragments. Mol Biotechnol 6, 287–310 (1996). https://doi.org/10.1007/BF02761708

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