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Peroxidase activity, isoenzymes and histological localisation in sapwood and heartwood of Scots pine (Pinus sylvestris L.)

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Journal of Forest Research

Abstract

Peroxidase activity and isoenzymes of fresh wood samples of the third shoot of 12-year old trees and from the sapwood, transition zone and heartwood of c. 60-year old stems of Scots pine (Pinus sylvestris L.) were investigated. Wood samples were ground at −30°C, extracted, and the extracts concentrated c. 20-fold for peroxidase activity assays (guaiacol method) and for IEF-PAGE. At least 11 major isoenzymes could be found in the gels. Even the heartwood contained some peroxidase isoenzymes. Isoenzyme patterns of the juvenile wood did not change with the season. However, juvenile wood showed the highest peroxidase activity at the end of the growing season. Peroxidase activity decreased from the outer sapwood towards the heartwood. Thin sections of different wood zones stained for peroxidase revealed activity in ray parenchyma and resin canal epithelial cells. Intensive staining was localised in the bordered pits of vertical and ray tracheids, and in the end walls of ray parenchyma cells.

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Authors and Affiliations

  1. Department of Biosciences, Division of Plant Physiology, University of Helsinki, P.O. Box 56, FIN-00014, Helsinki University, Finland

    Kurt Fagerstedt

  2. Finnish Forest Research Institute, P.O. Box 18, FIN-01301, Vantaa, Finland

    Pekka Saranpää & Riikka Piispanen

Authors
  1. Kurt Fagerstedt
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  2. Pekka Saranpää
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  3. Riikka Piispanen
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Fagerstedt, K., Saranpää, P. & Piispanen, R. Peroxidase activity, isoenzymes and histological localisation in sapwood and heartwood of Scots pine (Pinus sylvestris L.). J For Res 3, 43–47 (1998). https://doi.org/10.1007/BF02760292

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  • DOI: https://doi.org/10.1007/BF02760292

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