Skip to main content
SpringerLink
Log in

Comparative study of effects of cadmium cations in free and chelated forms on activity of glutathione S-transferase, growth, and endocytosis in culture of the infusoriumTetrahymena pyriformis

  • Comparative and Ontogenic Biochemistry
  • Published:
Journal of Evolutionary Biochemistry and Physiology Aims and scope Submit manuscript

Abstract

Effects of cadmium cations in free (Cd2+) and chelated with EDTA (Cd2+-EDTA) forms were studied on growth, endocytosis, and activity of glutathione S-transferase (GT) in the free-living infusoriaTetrahymena pyriformis. It is shown that the cytotoxicity of Cd2+ in the free form at a concentration of 10 μM is much higher than of the Cd2+-EDTA complex at the equimolar concentration. Even at a low concentration (2 μM), Cd2+ produces an inhibition of the growth rate and endocytosis in theT. pyriformis culture, while the Cd2+-EDTA complex suppresses these functions insignificantly. Cd2+ in the free form at concentrations of 10 and 100 μM reduced activity of glutathione S-transferase by 39 and 61%. The chelated Cd2+-EDTA complex at these concentrations inhibited the GT activity by 5 and 55%, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Nilsson, J.K., Tetrahymena in Cytotoxicology with Special Reference to Effects of Heavy Metals and Selected Drugs,Eur. J. Protistol., 1989, vol. 25, pp. 2–25.

    Google Scholar 

  2. Carter, J.W. and Cameron, I.L., Toxicity Bioassay of Heavy Metals in Water UsingTetrahymena pyriformis, Water Res., 1973, vol. 7, pp. 951–961.

    Article  CAS  Google Scholar 

  3. Hutner, S.H. and Marcus, S.L., Protozoological Approaches to the Cellular Basis of Mammalian Stress Repair,Int. Rev. Cytol., 1987, vol. 100, pp. 371–425.

    Article  PubMed  CAS  Google Scholar 

  4. Rana, S.V.S., Species Differences in Glutathione-Dependent Enzymes in the Liver and their Implications for Cadmium Toxicity,Ichthyological Res., 1996, vol. 43, pp. 223–229.

    Article  Google Scholar 

  5. Korotkov, S.M., Glasunov, V.V., Rozengart, E.V.,et al., Action of Organic Cadmium Complexes of Different Degree of Hydrophobicity on Rat Liver Mitochondria,Tsitologiya, 1996, vol. 38, pp. 1075–1083.

    CAS  Google Scholar 

  6. Dunlop, S. and Chapman, G., Detoxication of Zinc and Cadmium by Freshwater ProtozoanTetrahymena pyriformis. II. Growth Experiments and Ultrastructural Studies on Sequestration of Heavy Metals,Environ. Res., 1981, vol. 24, pp. 264–274.

    Article  CAS  Google Scholar 

  7. Houba, C., Kemacle, J., and De Parmetier, F., Influence of Cadmium onTetrahymena pyriformis in Axenic Culture,Eur. J. Appl. Microbiol. Biotechnol., 1981, vol. 11, pp. 179–182.

    Article  CAS  Google Scholar 

  8. Jacobson, K.B. and Turner, J.E., The Interaction of Cadmium and Certain Other Metal Ions with Proteins and Nucleic Acids,Toxicology, 1980, vol. 16, pp. 1–37.

    Article  PubMed  CAS  Google Scholar 

  9. Pyne, C.K., The Effects of Cadmium on the Growth Pattern and Ultrastructure of the CiliateTetrahymena pyriformis, and Antagonistic Effect of Calcium,Biol. Cell, 1983, vol. 48, pp. 121–132.

    CAS  Google Scholar 

  10. Vasconcelos, S.D.M.T., Azenha, M.A.O., and Cabrai, J.P.S., Comparison of Availability of Copper (II) Complexes with Organic Ligands to Bacterial Cell and to Chitin,Microbiology, 1997, vol. 16, pp. 2029–2039.

    CAS  Google Scholar 

  11. Habig, W.H., Pabst, M.I., and Jacoby, W.B., Glutathione S-Transferases: the First Enzymatic Step in Mercapturic Acid Formation,J. Biol. Chem., 1974, vol. 249, pp. 7130–7139.

    PubMed  CAS  Google Scholar 

  12. Lowry, O.N., Rosebrought, N.J., Farr, A.L., and Randall, R.J., Protein Measurement with the Folin Phenol Reagent,J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    PubMed  CAS  Google Scholar 

  13. Piccini, E., Irato, P., and Guidolin, L., Cadmium Thionein inTetrahymena thermophila andTetrahymena pyriformis, Eur. J. Protistol., 1990, vol. 26, pp. 176–181.

    Google Scholar 

  14. Cherian, M.G. and Nordberg, M., Cellular Adaptation in Metal Toxicology and Metallothionein,Toxicology, 1983, vol. 28, pp. 1–15.

    Article  PubMed  CAS  Google Scholar 

  15. Larsen, J. and Nilsson, J.P., Effects of Nickel on the Rates of Endocytosis, Mobility and Proliferation in Tetrahymena and Determination on the Cell Content of the Metal,Protoplasma, 1983, vol. 118, pp. 140–147.

    Article  CAS  Google Scholar 

  16. Mannervik, B., Alin, P., Guthenberg, C.,et al., Identification of Three Classes of Cytosolic Glutathione Transferase Common to Several Mammalian Species: Correlation between Structural Data and Enzymatic Properties,Proc. Natl Acad. Sci. USA, 1985, vol. 85, pp. 7202–7206.

    Article  Google Scholar 

  17. Dierickx, P.J.,In vitro Inhibition of the Soluble Glutathione S-Transferases from Rat Liver by Heavy Metals,Enzyme, 1982, vol. 27, pp. 25–32.

    PubMed  CAS  Google Scholar 

  18. Clark, A.C. and Revie, W., The Comparative Enzymology of the Glutathione S-Transferases from Nonvertebrate Organisms,Comp. Biochem. Physiol. B, 1989, vol. 92, pp. 419–446.

    Article  PubMed  CAS  Google Scholar 

  19. Dierickx, P.J., Glutathione S-Transferase in Aquatic Macroinvertebrates and Its Interaction with Different Organic Micropollutants,Sci. Total Environment, 1984, vol. 40, pp. 93–108.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    I. V. Shemarova, E. B. Maizel’ & A. E. Khovanskikh

Authors
  1. I. V. Shemarova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. B. Maizel’
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. E. Khovanskikh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shemarova, I.V., Maizel’, E.B. & Khovanskikh, A.E. Comparative study of effects of cadmium cations in free and chelated forms on activity of glutathione S-transferase, growth, and endocytosis in culture of the infusoriumTetrahymena pyriformis . J Evol Biochem Phys 36, 111–117 (2000). https://doi.org/10.1007/BF02754322

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02754322

Keywords

Search

Navigation