Abstract
In vitro selection of aptamers requires the reliable enzymatic preparation of large amounts of (+) single-stranded DNA molecules. This can be achieved by selective enzymatic digest of 5′-phosphorylated (−) strands from PCR products, a method already widely used in sequencing of PCR products. Here we present an adaptation of this method to prepare large pools of single-stranded DNA molecules for in vitro selection.
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Kujau, M.J., Wölfl, S. Efficient preparation of single-stranded DNA for in vitro selection. Mol Biotechnol 7, 333–335 (1997). https://doi.org/10.1007/BF02740823
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DOI: https://doi.org/10.1007/BF02740823