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Investigation of the presence of apolipoprotein E, glycosaminoglycans, basement membrane proteins, and protease inhibitors in senile interstitial amyloid of the pituitary

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Abstract

The aim of our study was to test whether local- or organ-limited interstitial amyloid of the pituitary is associated with the presence of glycosaminoglycans, basement membrane proteins, protease inhibitors, and apolipoprotein E (apo E), as previously observed in other amyloid syndromes. Serial sections from amyloidotic and nonamyloidotic autopsy pituitaries of patients age 85 yr and over were stained with Congo red, Alcian blue, and, applying immunohistochemistry, with antibodies directed against fibronectin, collagen IV, laminin, apo E, α1-antitrypsin and α1-antichymotrypsin. Interstitial amyloid was deposited in the immediate vicinity of capillaries and around the acini of the anterior lobe. Glycosaminoglycans were found in capillaries and around the acini of both nonamyloidotic and amyloidotic glands and they were also related spatially to amyloid deposits. Immunostatining of nonamyloidotic and amyloidotic glands demonstrated the presence of fibronectin, collagen IV, and laminin, which was related to basement membranes (fibronectin, collagen IV, and laminin), interstitium, and serum (fibronectin only). In amyloidotic glands, each basement membrane protein presented with an additional spatial relationship to amyloid deposits. Apo E was found in amyloidotic cases only within the amyloid deposits. The results are consistent with the presence of glycosaminoglycans, basement membrane proteins, and apo E in local interstitial amyloid deposits of the pituitary, as previously described in other amyloid syndromes, such as inflammatory related AA-amyloidosis or Aβ-amyloidosis related to Alzheimer’s disease.

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Authors and Affiliations

  1. Institute of Pathology, University of Magdeburg, Magdeburg, Germany

    Christoph Röcken MD

  2. Institute of Pathology, Marienkrankenhaus, Alfredstrasse 9, D-22087, Hamburg, Germany

    Diana Paris, Karen Steusloff & Wolfgang Saeger MD

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  1. Christoph Röcken MD
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  2. Diana Paris
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  3. Karen Steusloff
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  4. Wolfgang Saeger MD
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Röcken, C., Paris, D., Steusloff, K. et al. Investigation of the presence of apolipoprotein E, glycosaminoglycans, basement membrane proteins, and protease inhibitors in senile interstitial amyloid of the pituitary. Endocr Pathol 8, 205–214 (1997). https://doi.org/10.1007/BF02738787

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