Abstract
In the present study we investigated the effect of a long-term estrogen treatment on the intracellular distribution of VIP immunoreactivity in pituitary prolactin cells using double-labeling immunocytochemistry. With the use of pre-embedding ABC method it was found that VIP immunoreactivity was associated with the outer surface of membrane-bound organelles, and was not found in secretory granules. However, prolactin immunoreactivity demonstrated by postembedding immunogold technique was mainly associated within the secretory granules of the same cells. The discrepancy between our and Hsu et al.'s results (1989), who observed VIP immunoreactivity in secretory granules of human anterior pituitary cells, may be owing to the overstimulation of VIP cells by estrogen. It is possible that estrogen treatment depleted the VIP content of the secretory granules and enhanced the cytosolic VIP. The appearance of an alternative form of VIP in estrogen-treated rats with preferential distribution in the cytosol cannot be excluded.
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Arnaout, M. A., Garthwaite, T. L., Martyinson, D. R., and Hagen, T. C. (1986).Endocrinology 119, 2052–2057.
Carretero, J., Rubio, M., Navarro, N. N., Prieto, P., Vázquez, J. R., Sánchez, F., and Vazquez, R. (1995).Neuroendocrinology 62, 277–282.
Card, P., Brecha, N., Karten, J. G., and Moore, R. Y. (1981).J. Neurosci. 1, 1289–1303.
Carillo, A. J. and Phelps, C. J. (1992).Endocrinology 131, 964–969.
Chew, L. J., Murphy, D., and Carter, D. A. (1994).Mol. Endocrinol. 8, 603–613.
Childs, G. V. (1986).Curr. Top. Neuroendocrinol. 7, 49–97.
Gulyás, A., Gorcs, T. J., and Freund, T. (1990).Neuroscience 37, 31–44.
Hagen, T. C., Arnaout, I. M. A., Scherzer, W. J., Martinson, D. R., and Garthwaite, T. C. (1986).Neuroendocrinology 43, 641–45.
Hsu, D. W., Riskind, P. N., and Hedley-Whyte, E. T. (1989).Am. J. Pathol. 135, 329–38.
Köves, K., Gottschall, P. E., Gorcs, T. G., Scammell, J. G., and Arimura, A. (1990).Endocrinology 126, 1756–1763.
Lam, K. S. L., Srivastava, G., Lechan, R. M., Lee, T., and Reichlin, S. (1990).Neuroendocrinology 52, 417–421.
Lam, K. S. L., Lechan, R. M., Minamitani, N., Segerson, T. P., and Reichlin, S. (1989).Endocrinology 124, 1077–1084.
Larsson, L.-I., Fahrenkrug, J., Schaffalitzky de Muckadell, O., Sundler, F., Hakanson, R., and Rehfeld, J. F. (1976).Proc. Natl. Acad. Sci. USA 73, 3197–200.
Léránth, A., Köves, K., Chen, I.-L., Gottschall, P. G., Görcs, T. J., Scammell, J. G., and Arimura A. (1991).J. Endocrinol. Invest. 14(Suppl. 4), 205.
Lloyd, R. V. (1983).Am. J. Pathol. 113, 198–206.
Maletti, M., Rostene, W. H., Carr, L., Scherrer, H., Rotten, D., Kordon, C., and Rosselin, G. (1982).Neurosci. Lett. 32, 307–313.
Merchant, F. W. (1974).Am. J. Anat. 139, 245–268.
Morel, G., Besson, J., Rosselin, G., and Dubois, R. M. (1982).Neuroendocrinology 34, 85–89.
Nagy, G., Mulchahey, J. J., and Neill, J. D. (1988).Endocrinology 122, 364–366.
Nakane, P. K. (1975). In:The Anterior Pituitary. Farquhar, M. G. and Tixier-Vidal, A. (eds.). Academic: New York, pp. 45–61.
Nogami, H. (1984).Cell Tiss. Res. 237, 195–202.
O'Halloran, D. J., Jones, P. M., Ghatei, M. A., Domin, J., and Bloom, S. R. (1990).Endocrinology 127, 1463–69.
Ozawa, H. and Kurosumi, K. (1989). In:Proceedings of the Kyoto Prolactin Conference, vol. 4 Hoshino, K. (ed.). Shinko: Kyoto, pp. 58–67.
Prysor-Jones, R. A., Silverlight, J. J., Kennedy, S. J., and Jensins, J. S. (1988).J. Endocrinol. 116, 259–65.
Reichlin, S. S. I. (1988). In:Vasoactive Intestinal Peptide and Related Peptides. Said and Mutt, V. (eds.).Annals of the New York Academy of Sciences, vol. 257. New York Academy of Sciences: New York, pp. 431–449.
Rotsztejn, W. H., Besson, J., Brian, L., Gagnant, L., Rosselin, G., and Kordon, C. (1980).Neuroendocrinology 31, 287–91.
Said, S. T. and Mutt, V. (1970).Science 169, 1217–1218.
Said, S. T. and Rosenberg, R. N. (1976).Science 192, 907,908.
Scammell, J. G., Scott, M. G., Outlaw, K. K., Thompson, M. E., OS-J, and Belen, R. B. (1990).J. Histochem. Cytochem. 38, 117–122.
Shiino, M. and Rennels, E. G. (1976).Anat. Rec. 185, 31–48.
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Köves, K., Chen, IL., Görcs, T.J. et al. Different ultrastructural localization of VIP and prolactin in anterior pituitary cells of rats chronically treated with estrogen. Endocr 5, 219–223 (1996). https://doi.org/10.1007/BF02738709
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DOI: https://doi.org/10.1007/BF02738709