Skip to main content
SpringerLink
Log in

Endometrial ISG17 mRNA and a related mRNA are induced by interferon-tau and localized to glandular epithelial and stromal cells from pregnant cows

  • Original Articles
  • Published:
Endocrine Aims and scope Submit manuscript

Abstract

The interferon stimulated gene product, ISG17, conjugates to bovine uterine proteins in response to conceptus-derived interferon (IFN)-τ. The objectives of the present experiments were to examine induction of ISG17 (0.65 kb) and a related 2.5 kb mRNA in response to IFN-τ and pregnancy using Northern blotting procedures, and to determine cell types in the endometrium that expressed ISG17 mRNA usingin situ hybridization. RNA was isolated from endometrial explants or from bovine endometrial (BEND) cells cultured in the absence (control) or presence of 25 nM recombinant (r) bolFN-τ for 0, 3, 6, 12, 24, or 48 h. The major ISG17 0.65 kb mRNA and a minor 2.5 kb mRNA were induced (p<0.05) after 6 h (explants) or 3 h (BEND cells) treatment with rbolFN-τ. Both mRNAs were present in endometrium from day 18 pregnant cows, but were absent in endometrium from nonpregnant cows. The ISG17 mRNA was localized to stromal and glandular epithelial cells on d 18 of pregnancy. The 2.5 kb mRNA may encode a novel ISG17 homolog, or a unique polyISG17 repeat that is similar in structure to the polyubiquitin genes. Because ISG17 mRNA is induced in stromal and glandular epithelial cells, it could be assumed that ISG17 has a role in regulating intracellular proteins in both cell types.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Farrell, P. J., Broeze, R. J., and Lengyel, P. (1979).Nature 279, 523–525.

    Article  PubMed  CAS  Google Scholar 

  2. Haas, A. L., Ahrens, P., Bright, P. M., and Ankel, H. (1987).J. Biol. Chem. 262, 11,315–11,323.

    CAS  Google Scholar 

  3. Knight, Jr., E. and Cordova, B. (1991).J. Immunol. 146, 2280–2284.

    PubMed  CAS  Google Scholar 

  4. Loeb, K. R. and Haas, A. L. (1992).J. Biol. Chem. 267, 7806–7813.

    PubMed  CAS  Google Scholar 

  5. Blomstrom, D. C., Fahey, D., Kutny, R., Korant, B. D., and Knight, E., Jr. (1986).J. Biol. Chem. 261, 8811–8816.

    PubMed  CAS  Google Scholar 

  6. Korant, B. D., Blomstrom, D. C., Jonak, G. J., and Knight, E., Jr. (1984).J. Biol. Chem. 259, 14,835–14,839.

    CAS  Google Scholar 

  7. Recht, M., Borden, E. C., and Knight, E. Jr. (1991).J. Immunol. 147, 2617–2623.

    PubMed  CAS  Google Scholar 

  8. D'Cunha, J. D., Knight, Jr., E., Haas, A. L., Truitt, R. L., and Borden, E. C. (1996).Proc. Natl. Acad. Sci. USA 93, 211–215.

    Article  Google Scholar 

  9. D'Cunha, J. D., Ramanujam, S., Wagner, R. J., Witt, P. L., Knight, Jr., E., and Borden, E. C. (1996).J. Immunol. 157, 4100–4108.

    Google Scholar 

  10. Knight, E. Jr., Fahey, D., Cordova, B., Hollman, M., Kutny, T., Reich, N., and Blomstrom, D. C. (1988).J. Biol. Chem. 263, 4520–4522.

    PubMed  CAS  Google Scholar 

  11. Reich, N., Evans, D., Levy, D., Fahey, D., Knight, E., Jr., and Darnell, J. E., Jr. (1987).Proc. Natl. Acad. Sci. USA 84, 6394–6398.

    Article  PubMed  CAS  Google Scholar 

  12. Ecker, D. J., Butt, T. R., Marsh, J., Sternberg, E. J., Margolis, N., Monia, B. P., Jonnalagadda, S., Khan, M. I., Weber, P. L., Mueller, L., and Crooke, S. T. (1987).J. Biol. Chem. 262, 14,213–14,221.

    CAS  Google Scholar 

  13. Wilkinson, K. D. and Audhya, T. K. (1981).J. Biol. Chem. 256, 9235–9241.

    PubMed  CAS  Google Scholar 

  14. Narasimhan, J., Potter, J. L., and Haas, A. L. (1996).J. Biol Chem 271, 324–330.

    Google Scholar 

  15. Lowe, J., Mcdermott, H., Loeb, K., Landon, M., Haas, A. L., and Mayer, R. J. (1995).J. Pathology 177, 163–169.

    Article  CAS  Google Scholar 

  16. Loeb, K. R. and Haas, A. L. (1994).Molec Cell Biol 14, 8408–8419.

    PubMed  CAS  Google Scholar 

  17. Bebington, C., Bell, S., Doherty, F., Fazleabas, A., and Fleming, S. (1999).Biol Reprod In press.

  18. Finley, D. and Chau, V. (1991).Annul. Rev. Cell. Biol. 7, 25–69.

    CAS  Google Scholar 

  19. Hershko, A. and Ciechanover, A. (1992).Annul. Rev. Biochem. 61, 761–807.

    Article  CAS  Google Scholar 

  20. Finley, D., Ciechanover, A., and Varshavsky, A. (1984).Cell 37, 43–55.

    Article  PubMed  CAS  Google Scholar 

  21. Hicke, L. (1997).FASEB 11, 1215–1226.

    CAS  Google Scholar 

  22. Kim, T. K., and Maniatis, T. (1996).Science 273, 1717–1719.

    Article  Google Scholar 

  23. Palombella, V. J., Rando, O. J., Goldberg, A. L., and Maniatis, T. (1994).Cell 78, 773–785.

    Article  PubMed  CAS  Google Scholar 

  24. Naivar, K. A., Ward, S. K., Austin, K. J., Moore, D. W., and Hansen, T. R. (1995).Biol. Reprod. 52, 848–854.

    Article  PubMed  CAS  Google Scholar 

  25. Austin, K. J., Ward, S. K., Teixeira, M. G., Dean, V. C., Moore, D. W., and Hansen, T. R. (1996).Biol. Reprod. 54, 600–606.

    Article  Google Scholar 

  26. Austin, K. J., Pru, J. K., and Hansen, T. R. (1996).Endocrine 5, 1–7.

    Google Scholar 

  27. Johnson, G. A., Austin, K. J., Van Kirk, E. A., and Hansen, T. R. (1998).Biol. Reprod. 58, 898–904.

    Article  PubMed  CAS  Google Scholar 

  28. Jentsch, S., Seufert, W., and Hauser, H. P. (1991).Biochim Biophys Acta 1089, 127–139.

    PubMed  CAS  Google Scholar 

  29. Baker, R. T. and Board, P. G. (1981).Nucleic Acids Res. 19, 1035–1040.

    Article  Google Scholar 

  30. Redman, K. L. and Burris, G. W. (1996).Biochem. J. 315, 315–321.

    Google Scholar 

  31. Finley, D., Bartel, B., and Varshavsky, A. (1989).Nature 338, 394–401.

    Article  PubMed  CAS  Google Scholar 

  32. Baker, R. T. and Board, P. G. (1989).Am. J. Hum. Genet. 44, 534–542.

    PubMed  CAS  Google Scholar 

  33. Perry, D. J., Austin, K. J., and Hansen, T. R. (1999).Molecular Endocrinology (In Press).

  34. Hansen, T. R., Austin, K. J., and Johnson, G. A. (1987).Endocrinology 138, 5079–5082.

    Article  Google Scholar 

  35. Thatcher, W. W., Meyer, M. D. and Danet-Desnoyers, G. (1995).J. Reprod. Fertil. 49, 15–28.

    CAS  Google Scholar 

  36. Roberts, R. M., Cross, J. C., and Leaman, D. W. (1990).Endocrine Reviews 13, 432–452.

    Article  Google Scholar 

  37. Haas, A. L. and Siepmann, T. J. (1997).FASEB 11, 1257–1268.

    CAS  Google Scholar 

  38. Johnsson, N. and Varshavsky, A. (1994).EMBO J. 13, 2686–2698.

    PubMed  CAS  Google Scholar 

  39. Haas, A. L. and Bright, P. M. (1987).J. Biol. Chem. 262, 345–351.

    PubMed  CAS  Google Scholar 

  40. Bond, U. and Schlesinger, M. J. (1985).Mol. Cell. Biol. 5, 949–956.

    PubMed  CAS  Google Scholar 

  41. Staggs K. L., Austin, K. A., Johnson, G. A., Teixeira, M. G., Talbott, C. T., Dooley, V. A., and Hansen, T. R. (1998).Biol. Reprod 59, 293–297.

    Article  PubMed  CAS  Google Scholar 

  42. Murdoch, W. J., Austin, K. J., and Hansen, T. R. (1996).Endocrinology 137, 4526–4529.

    Article  Google Scholar 

Download references

Author information

Author notes

  1. Gregory A. Johnson

    Present address: Department of Animal Science, Texas A&M University, 77843-2471, College Station, TX

Authors and Affiliations

  1. Reproductive Biology Program, Department of Animal Science, University of Wyoming, 82071-3684, Laramie, WY

    Gregory A. Johnson, Kathy J. Austin, Alisha M. Collins, William J. Murdoch & Thomas R. Hansen

Authors
  1. Gregory A. Johnson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kathy J. Austin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alisha M. Collins
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. William J. Murdoch
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Thomas R. Hansen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Johnson, G.A., Austin, K.J., Collins, A.M. et al. Endometrial ISG17 mRNA and a related mRNA are induced by interferon-tau and localized to glandular epithelial and stromal cells from pregnant cows. Endocr 10, 243–252 (1999). https://doi.org/10.1007/BF02738623

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02738623

Key Words

Search

Navigation