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The influence of antiadhesion therapies on leukocyte subset accumulation in central nervous system ischemia in rats

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Abstract

Although treatment with agents that block leukocyte function, including anti-ICAM-1 and doxycycline, reduces experimental central nervous system (CNS) ischemic injury, it is not known how leukocyte subset accumulation is affected by these agents. Using the rat two-vessel occlusion model and immunohistochemistry, we investigated granulocyte (PMN) and monocyte/macrophage (Mφ) accumulation at 1 and 4 d postischemia. A total of 24 animals were randomized to sham surgery, or to ischemia with saline, anti-ICAM-1, or doxycycline treatments. No leukocytes were observed in sham animals. At 24 h postischemia, there was a moderate infiltration of PMN and Mφ in untreated animals that was significantly decreased with either treatment. At 4 d after ischemia no PMN were identified, with extensive Mφ accumulation occurring in untreated animals that was only partially reduced with doxycycline treatment. These results confirm that both anti-ICAM-1 and doxycycline treatments reduce PMN and Mφ infiltration at 24 h. Delayed Mφ accumulation occurs despite treatment, suggesting that some of these cells represent transformed resident microglia.

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Authors and Affiliations

  1. Oregon Stroke Center, Department of Neurology, Oregon Health Sciences University, Portland, OR

    Wayne M. Clark, Jeff D. Lauten, Nikola Lessov, William Woodward & Bruce M. Coull

  2. Department of Neurology, Veterans Administration Medical Center, Portland, OR

    Bruce M. Coull

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  1. Wayne M. Clark
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  2. Jeff D. Lauten
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  3. Nikola Lessov
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  4. William Woodward
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  5. Bruce M. Coull
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Clark, W.M., Lauten, J.D., Lessov, N. et al. The influence of antiadhesion therapies on leukocyte subset accumulation in central nervous system ischemia in rats. J Mol Neurosci 6, 43–50 (1995). https://doi.org/10.1007/BF02736758

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  • DOI: https://doi.org/10.1007/BF02736758

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