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Biochemical and morphological characterization of potato clones differing in their resistance to late blight

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Summary

Morphological and biochemical parameters that could be involved in resistance to late blight were studied in non-infected and in infected potato hybrids resulting from a cross betweenSolanum phureja, resistant toPhytophthora infestans, and a susceptibleSolanum tuberosum.

Some morphological differences between resistant and susceptible hybrids, indicating a positive correlation between stem diameter and phloem thickness in the stem and resistance toP. infestans, were observed. The lignin content in the leaves of the resistant hybrid rose upon infection byP. infestans. In the leaves of the susceptible hybrid, a diminution of the lignin content could be observed upon infection byP. infestans. In the same context, peroxidasic activity raised upon infection byP. infestans in both resistant and susceptible hybrids. Further characterization of the hybrid clones based on the polymorphism of peroxidases was attempted using isoelectric focusing.

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References

  • Agrios, G.N., 1997. Plant Pathology. Academic Press Limited, London, pp. 93–114.

    Google Scholar 

  • Collins, A., D. Milbourne, L. Ramsay, R. Meyer, C. Chatot-Balandras, P. Oberhagemann, W. De Jong, C. Gebhardt, E. Bonnel & R. Waugh, 1999. QTL for field resistance to late blight in potato are strongly correlated with maturity and vigour.Molecular Breeding 5: 387–398.

    Article  CAS  Google Scholar 

  • Dowley, L.J., E. O’Sullivan & H.W. Kehoe, 1991. Development and evaluation of blight resistant potato cultivars. In: Lucas, J.A., R.C. Shattock, D.S. Shaw, L.R. Cooke (Eds), Phytophthora. Cambridge University Press, Cambridge, pp. 373–382.

    Google Scholar 

  • Enyedi, A.J., N. Yalpani, P. Silverman & I. Raskin, 1992. Signal molecules in systemic plant resistance to pathogens and pests.Cell 70: 879–886.

    Article  PubMed  CAS  Google Scholar 

  • Friend, J., S.B. Reynolds & M.A. Aveyard, 1973. Phenylalanine ammonia lyase, chlorogenic acid and lignin in potato tuber tissue inoculated with Phytophthora infestans.Physiological Plant Pathology 3: 495–507.

    Article  CAS  Google Scholar 

  • Ghislain, M., B. Trognitz, Ma. del R. Herrera, J. Solis, G. Casallo, C. Vásquez, O. Hurtado, R. Castillo, L. Portal & M. Orrillo, 2001. Genetic loci associated with field resistance to late blight in offspring ofSolanum phureja andS. tuberosum grown under short-day conditions.Theoretical and Applied Genetics 103: 433–442.

    Article  CAS  Google Scholar 

  • Giebel, J., 1982. Mechanisms of resistance to plant nematodes.Annual Review of Phytopathology 20: 257–279.

    Article  CAS  Google Scholar 

  • Hohl, H.R., 1989. Surface-related host-pathogen interactions inPhytophthora Phytophthora, Cambridge University Press, Cambridge.

    Google Scholar 

  • Kombrink, E., L. Beerhues, F. Garcia-Garcia, K. Hahlbrock, M. Müller, M. Schröder, B. Witte & E. Schmelzen, 1993. Expression patterns of defense-related genes in infected and uninfected plants. In: Fritig, B. & M. Legrand (Eds), Mechanism of Plant Defense Responses. Kluwer Academic Publishers.

  • Kovats, K., A. Binder & H.R. Hohl, 1991. Cytology of induced systemic resistance of cucumber to Colletotrichum lagenarium.Planta 183: 484–490.

    Google Scholar 

  • Lewis, N.G. & E. Yamamoto, 1990. Lignin: occurrence, biogenesis and biodegradation.Annual Review of Plant Physiology and Plant Molecular Biology 41: 455–496.

    Article  PubMed  CAS  Google Scholar 

  • Milosevic, N. & A.J. Slusarenko, 1996. Active oxygen metabolism and lignification in the hypersensitive response in bean.Physiological and Molecular Plant Pathology 49: 143–158.

    Article  Google Scholar 

  • Moerschbacher, B.M., U. Noll, L. Gorrichon & H.J. Reisener, 1990. Specific inhibition of lignification breaks hypersensitive resistance of wheat to stem rust.Plant Physiology 93: 465–470.

    Article  PubMed  CAS  Google Scholar 

  • Moncousin, C. & T. Gaspar, 1983. Peroxidase as a marker for rooting improvement ofCyna-ra scolymus L. cultured in vitro.Biochemie und Physiologie der Pflanzen 178: 263–271.

    CAS  Google Scholar 

  • Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture.Physiologia Plantarum 15: 473–497.

    Article  CAS  Google Scholar 

  • Nagel, L.M., J.H. Bassman, G.E. Edwards, R. Robberecht & V.R. Franceshi, 1998. Leaf anatomical changes inPopulus trichocarpa, Quercus rubra, Pseudotsuga menziesii andPinus ponderosa exposed to enhanced ultraviolet-B radiation.Physiologia Plantarum 104: 385–396.

    Article  CAS  Google Scholar 

  • Nicholson, R.L., 1984. Adhesion of fungi to plant cuticles. In: Roberts, D.W. & J.R. Aist (Eds), Infection Processes of Fungi. Rockefeller Foundation, New York.

    Google Scholar 

  • Oberhagemann, P., C. Chatot-Balandras, R. Schäfer-Pregl, D. Wegener, C. Palomino, F. Salimini, E. Bonnel & C. Gebhardt, 1999. A genetic analysis of quantitative resistance to late blight in potato: towards marker-assisted selection.Molecular Breeding 5: 399–415.

    Article  CAS  Google Scholar 

  • Reuveni, M., 1998. Relationships between leaf age, peroxidase and β-1,3-glucanase activity, and resistance to downy mildew in grapevines.Journal of Phytopathology 146: 525–530.

    Google Scholar 

  • Spector, T., 1978. Refinement of the Coomassie blue method for protein quantitation.Analytical Biochemistry 86: 142–146.

    Article  PubMed  CAS  Google Scholar 

  • Umaeras, V., 1959. Relationship between peroxidase activity in potato leaves and resistance to Phytophthora infestans.American Potato Journal 36: 124–131.

    Google Scholar 

  • Valette, C, C. Andary, J.P. Geiger, J.L. Sarah & M. Nicole, 1998. Histochemical and cytochemical investigations of phenols in roots of banana infected by the burrowing nematode Radopholus similis.Phytopathology 88: 1141–1148.

    Article  CAS  PubMed  Google Scholar 

  • Vance C.P., T.K. Kirk & R.T. Sherwood, 1980. Lignification as a mechanism of disease resistance.Annual Review of Phytopathology 18: 259–288.

    Article  CAS  Google Scholar 

  • Vez, A., 1994. Apport de l’électrophorèse dans l’identification des variétés de pomme de terre cultivées en Suisse.Revue Suisse Agricole 26: 373–379.

    Google Scholar 

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Evers, D., Welschbillig, N., Dommes, J. et al. Biochemical and morphological characterization of potato clones differing in their resistance to late blight. Potato Res 46, 105–115 (2003). https://doi.org/10.1007/BF02736080

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