Abstract
The embryonic and larval development ofCobitis takatsuensis, a mountain stream spinous loach, was surveyed by incubating artificially inseminated eggs. The mean diameter of the inflated eggs and mean total length of newly-hatched larvae were 2.7 mm and 5.7 mm, respectively. The eggs were spherical, transparent and unpigmented, with a pale yellow yolk and no oil globule. The daily cumulative temperature to hatching was estimated to be 70–110°C. day. Hatched larvae were unpigmented with outer gill filaments on their cheeks, as in otherCobitis species, but the melanophores were comparatively less obvious at each developmental stage. The larvae started feeding eleven days after hatching yolk absorption being completed sixteen days after hatching. All the fin rays were fully developed and the juvenile stage reached at 16 mm TL, 38 days after hatching. Embryonic and larval developmental traits ofC. takatsuensis, such as egg size, clutch size and larval pigmentation, were similar to the Korean species,Niwaella multifasciata, that lives in the upper reaches of the Nak-tong river, andN. delicata, which inhabits Japanese mountain streams, rather than to its congeners. Among cobitine fishes, the spawning of a small number of larger eggs yielding larger larvae without pigmentation, characteristics shared byC. takatsuensis, N. multifasciata andN. delicata, is attributable to adaptation to cold mountain streams.
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Literature Cited
Goto, A. 1977. Some considerations on speciation and adaptation of the freshwater sculpins in Hokkaido, Japan. J. Michurin Biol., 13: 39–47. (In Japanese with English summery.)
Honjo, T. and J. Taguchi, 1974. Studies on the reproduction of the delicate loach,Niwaella delicata—I. On the artificial spawning, hatching and rearing of delicate loach. Bull. Gifu Pref. Fish. Exp. Sta., (19): 1–7 (In Japanese.)
Hosoya, K. 1988a. Cobitididae. Pages 157in M. Okiyama, ed. An atlas of the early stage fishes in Japan. Tokai Univ. Press, Tokyo xii+1154 pp. (In Japanese.)
Hosoya, K. 1988b.Misgurnus anguillicaudatus. Page 159in M. Okiyama, ed. An atlas of the early stage fishes in Japan. Tokai Univ. Press, Tokyo. xii+1154 pp. (In Japanese.)
Kendall, Jr., A. W., E. H. Ahlstrom and H. G. Moser. 1984. Early life history stages of fishes and their characters. Pages 11–22in H. G. Moser, W. J. Richards, D. M. Chohen, M. P. Fahay, A. W. Kendall, Jr. and S. L. Richardson, eds. Ontogeny and systematics of fishes. Am. Soc. Ichthyol. Herpetol. Spec. Publ. 1.
Kim, I.-S. and W.-O. Lee. 1995. Studies on early embryonic development ofNiwaella multifasciata (Pisces: Cobitidae). Kor. J. Limn., 28: 455–462. (In Korean with English abstract.)
Kimizuka, Y., H. Kobayashi and N. Mizuno. 1982. Geographic distributions and karyotypes ofCobitis takatsuensis andNiwaella delicata (Cobitididae). Japan. J. Ichthyol., 29: 305–310.
Kishi, Y. 1978. How is the egg-size determined? Biol. Sci. (Tokyo), 30: 48–56. (In Japanese.)
Kubota, Z. and I. Matsui, 1954. Influence of temperature on the embryonic development of the loach,Misgurnus anguillicaudatus (Cantor). J. Shimonoseki Coll. Fish., 3: 209–215. (In Japanese with English summery.)
Minamori, S. 1951. Hybridization and classification in spinous loaches. Japan. J. Ichthyol., 1: 215–225. (In Japanese.)
Minamori, S. 1955. Temperature adaptation of some local races of striated spinous loach. Japan. J. Ecol., 4: 66–68. (In Japanese with English summery.)
Minamori, S. 1960. Speciation—a case of the striated spinous loach. Biol. Sci. (Tokyo), 12: 72–74. (In Japanese.)
Mizuno, N. 1963. Distribution ofCottus japonicus Okada (Cottidae) andTukugobius flumineus Mizuno (Gobidae), with special references to their peculiarities in both the land-locking and the speciation from amphidromous ancestors. Bull. Osaka Gakugei Univ., (11): 129–161. (In Japanese with English summery).
Mizuno, N. 1975. On the notes ofCobitis takatsuensis. The Freshwater Fishes, (1): 80–82. (In Japanese.)
Mori, S., M. Okazaki and T. Honjo 1975. Studies on the reproduction of the delicate loach,Niwaella delicata. —III. On the spawning in artificial construction of stones and the rearing of juvenile fishes in ponds. Bull. Gifu Pref. Fish. Exp. Sta., (20): 21–26, (In Japanese.)
Mori, S. and J. Taguchi, 1975. Studies on the reproduction of the delicate loach,Niwaella delicata.—V. On the structure of the spawning bed. Bull. Gifu Pref. Fish. Exp. Sta., (20): 35–39. (In Japanese.)
Niwa, H. 1954. Fish of Kiso valley. Ecological Studies of the river fish fauna. Part I. Investigation of upper parts of the River Kiso and its tributaries. Kiso Kyoiku-kai, Nagano, xvi+302 pp. (In Japanese.)
Niwa, H. 1976. Adime Comprehensive studies onNiwaella delicata (Niwa), a Japanese species of Cobitidae. Taisyu-shubou, Gifu, 226 pp. (In Japanese.)
Okada, Y. and R. Seishi. 1937. Morphological and ecological study on the larvae and juveniles of freshwater fishes in Japan. (viii)Cobitis biwae Jordan & Snyder. Bull. Fish. Res., 32: 549–554 (In Japanese.)
Saitoh, K. 1990. Reproductive and habitat isolation between two populations of the striated spined loach. Env. Biol. Fish., 28: 237–248.
Saitoh, K. and K. Hosoya, 1988a.Cobitis biwae Jordan et Snyder. Page 159in M. Okiyama, ed. An atlas of the early stage fishes in Japan. Tokai Univ. Press, Tokyo. xii+1154 pp. (In Japanese.)
Saitoh, K. and K. Hosoya. 1988a.Cobitis sp. 1. Pages 159–161in M. Okiyama, ed. An atlas of the early stage fishes in Japan. Tokai Univ. Press, Tokyo. xii+1154 pp. (In Japanese.)
Saitoh, K. and K. Hosoya. 1988c.Cobitis sp. 2. Pages 161in M. Okiyama, ed. An atlas of the early stage fishes in Japan. Tokai Univ. Press, Tokyo, xii+1154 pp. (In Japanese.)
Saitoh, K. and K. Hosoya. 1988d.Cobitis sp. 3. Pages 163in M. Okiyama, ed. An atlas of the early stage fishes in Japan. Tokai Univ. Press, Tokyo, xii+1154 pp. (In Japanese.)
Saitoh, K. and M. Matsuda, 1990. An introduction to the spined loaches in the Lake Biwa basin. Ann. Rep. Biwako-Bunkakan, (8): 19–23. (In Japanese.)
Saitoh, K., O. Katano and A. Koizumi. 1988. Movement and spawning of several freshwater fishes in temporary waters around paddy fields. Japan. J. Ecol., 38: 35–47. (In Japanese with English synopsis.)
Sakai, H. 1990. Larval developmental intervals inTribolodon hakonensis (Cyprinidae). Japan. J. Ichthyol., 37: 17–28.
Sakai, H., T. Yokoyama, Y. Sakai, S. Matsumoto and Z. Kubota. 1989. Notes on the life history ofCobitis takatsuensis, a spinous loach. Bull. Biogeogr. Soc. Japan, 44: 39–47. (In Japanese with English abstract.)
Suzuki, R. 1966. Artificial spawning and early development of the loach,Cobitis delicata Niwa. Bull. Freshwater Fish. Res. lab., 15: 175–188. (In Japanese with English synopsis.)
Suzuki, R. 1976. Number of ovarian eggs and spawned eggs and their size composition in the loach, cyprinid fish. Bull. Japan. Soc. Sci. Fish., 42: 961–967. (In Japanese with English summery.)
Suzuki, R. 1983. The newest aquaculture technologies of loach. Taibunkan, Tokyo. 192 pp. (In Japanese.)
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Shimizu, T., Sakai, H. & Mizuno, N. Embryonic and larval development of a Japanese spinous loach,Cobitis takatsuensis . Ichthyol Res 45, 377–384 (1998). https://doi.org/10.1007/BF02725190
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DOI: https://doi.org/10.1007/BF02725190