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A new method for establishing an intrapulmonary tumor in the rabbit

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Abstract

Objectives: Local treatments against malignant intrapulmonary tumors, such as radiotherapy, photodynamic therapy, and laser ablation therapy, are sometimes used in preference to surgery or chemotherapy. The efficacy of such treatments is dependent not only on the pathological type of the primary tumor, but also on loco-regional settings such as location of the lesion and relationship between the tumor and surrounding organs as well as tissues. Therefore, in order to evaluate the efficacy of a newly developed local treatment, it is essential to have a model in which a tumor can be established at an intended location. In this study, we developed a model for the establishment of an intrapulmonary tumor, using the rabbit.Methods: After induction of general anaesthesia, a catheter was inserted via the airway into the lung of a Japanese wiite rabbit, under X-ray guidance. The lung was then inoculated with a collagen gel matrix containing a suspension of VX2 cells derived from rabbit skin squamous cell carcinoma. Subsequent tumor growth was evaluated with X-ray imaging and pathological examination.Results: Growth of a solitary tumor at the arget site was pathologically confirmed in 9 of 12 rabbits that were inoculated with 1×108 cells. In addition, tumor growth could be followed in 8 of 9 rabbits by chest X-ray examination. That is, a solitary intrapulmonary tumor could be established in 8 of 12 animals (67%).Conclusion: The rabbit model reported here enables establishment of a solitary intrapulmonary tumor and thus can provide a suitable experimental setting for evaluation of local treatments of intrapulmonary tumors.

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Authors and Affiliations

  1. Department of Thoracic and Cardiovascular Surgery, Kansai Medical University Hospital, 10-15 Fumizonocho, Moriguchi, 570-8506, Osaka, Japan

    Yuzo Shomura MD, Yukihito Saito MD, Kenichiro Minami MD & Hiroji Imamura MD

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  1. Yuzo Shomura MD
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  2. Yukihito Saito MD
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  3. Kenichiro Minami MD
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  4. Hiroji Imamura MD
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Shomura, Y., Saito, Y., Minami, K. et al. A new method for establishing an intrapulmonary tumor in the rabbit. Jpn J Thorac Caridovasc Surg 51, 337–343 (2003). https://doi.org/10.1007/BF02719464

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  • DOI: https://doi.org/10.1007/BF02719464

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