Abstract
Variation of the thermotropic behaviour of both lipid assemblies and associated water molecules with an increase in water content was investigated for negatively charged phosphatidyl-glycerol (PG)-water system up to 90 wt.% water by DSC. The number of water molecules existing in interbilayer regions of the present gel phase was estimated from a deconvolution analysis of ice-melting DSC curves. On the basis of a result of the calorimetric analysis, a water-distribution diagram was constructed over the water content range from 0 to 90 wt.%. The diagram presented a continuous incorporation of interlamellar water up to 90 wt.% water, related to unilamellar-vesicle forming properties of charged lipids. Furthermore, similarly to a result for neutral lipid systems previously reported by us, the present diagram also showed the existence of a specific water content region (i.e., pre-region) where a structural change of planar to curved bilayers for multilamellar structures proceeds with the aid of bulk-like water before finally reaching unilamellar vesicles.
Similar content being viewed by others
References
A. Watts, K. Harlos and D. Marsh, Biochim. Biophys. Acta, 645 (1981) 91.
J. K. Ranck and J. F. Tocanne, FEBS Lett., 143 (1982) 171.
J. M. Boggs and G. Rangaraj, Biochemistry, 22 (1983) 5425
H. Hauser, Biochim. Biophys. Acta, 772 (1984) 37.
D. A. Wilkinson, D. A. Tirrell, A. B. Turek and T. J. McIntosh, Biochim. Biophys. Acta, 905 (1987) 447.
M. Kodama and T. Miyata, Thermochim. Acta, 267 (1995) 365.
M. Kodama and T. Miyata, Colloid Surfaces A, 109 (1996) 283.
M. Kodama, T. Miyata and T. Yokoyama, Biochim. Biophys. Acta, 1168 (1993) 243.
9 D. Atkinson, H. Hauser, G. G. Shipley and J. M. Stubbs, Biochim. Biophys. Acta., 339 (1974) 10–29.
E. G. Finer and A. Darke, Chem. Phys. Lipids, 12 (1974) 1–16.
H. Hauser, F. Paltauf and G. G. Shipley, Biochemistry, 21 (1982) 1061–1067.
H. Aoki and M. Kodama, Thermochim. Acta, (in press)
M. Kodama, H. Aoki, H. Takahashi and I. Hatta, Biochim. Biophys. Acta, (in press).
M. Kodama, M. Kuwabara and S. Seki, Biochim. Biophys. Acta, 689 (1982) 567.
M. Kodama, T. Miyata and Y. Takaichi, Biochim. Biophys. Acta, 1169 (1993) 90.
M. Kodama, H. Inoue and Y. Tsuchida, Thermochim. Acta, 266 (1995) 373.
M. Kodama, Thermochim. Acta, 109 (1983) 81.
G. Klose, B. König, H. W. Meyer, G. Schulze and G. Degovics, Chem. Phys. Lipids, 47 (1988) 225.
J. F. Nagle, R. Zhang, S. Tristram-Nagle, W. Sun, H. I. Petrache and R. M. Suter, Biophys. J., 70 (1996) 1419.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kodama, M., Nakamura, J., Miyata, T. et al. The behaviour of water molecules associated with structural changes in negatively charged phosphatidyl-glycerol assemblies as studied by DSC. J Therm Anal Calorim 51, 91–104 (1998). https://doi.org/10.1007/BF02719013
Issue Date:
DOI: https://doi.org/10.1007/BF02719013