Abstract
We have studied the antagonism between insulin and dexamethasone on phosphatidylcholine synthesis in fetal rat lung in organ culture. Explants from 18-day fetal rat lungs were cultured in serum-free medium in the presence of 10−6 M dexamethasone phosphate, 1 U/ml bovine insulin or both hormones for 48 h. Control explants were similarly cultured in the absence of hormones. Dexamethasone alone stimulated the activity of cholinephosphate cytidylyltransferase by 134% while insulin alone had no effect. When insulin was added together with dexamethasone, however, the stimulation was reduced to 72%. These data suggest that antagonism by insulin of dexamethasone-induced stimulation of fetal lung phosphatidylcholine biosynthesis may be at least partially expressed at the level of cholinephosphate cytidylyltransferase.
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Supported by grants HD-11018 and HL-19752 from the National Institutes of Health, United States Public Health Service
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Rooney, S.A., Ingleson, L.D., Wilson, C.M. et al. Insulin antagonism of dexamethasone-induced stimulation of cholinephosphate cytidylyltransferase in fetal rat lung in organ culture. Lung 158, 151–155 (1980). https://doi.org/10.1007/BF02713717
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DOI: https://doi.org/10.1007/BF02713717