Abstract
Much of the modern understanding of orientational order in liquid crystals (LCs) is based on polarizing microscopy (PM). A PM image bears only two-dimensional (2D) information, integrating the 3D pattern of optical birefringence over the path of light. Recently, we proposed a technique to image 3D director patterns by fluorescence confocal polarizing microscopy (FCPM). The technique employs the property of LC to orient the fluorescent dye molecules of anisometric shape, added in small quantities to the LC. In LC, smooth director deformations do not alter mass density of the material. Thus the density of dye is also uniform across the sample, except, perhaps, near the surfaces or at the cores of topological defects. In polarized light, the measured fluorescence signal is determined by the spatial orientation of the molecules rather than by dye concentration (as in regular biological samples stained with tissue-specific dyes). The contrast is enhanced when both excitation and detection of fluorescence light are performed in polarized light. This short review describes the essence of FCPM technique and illustrates some of its applications, including imaging of Frederiks electric-field induced effect in a nematic LC and defects such as dislocations in cholesteric LCs.
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References
P G de Gennes and J Prost,The physics of liquid crystals, 1st edition (1974); 2nd edition (Oxford Univ. Press, Oxford, 1993) p. VII
N H Hartshorn,Microscopy of liquid crystals (Microscope Publications, London, 1974)
G H Meeten (ed.),Optical properties of polymers (Elsevier, Essex, 1986)
J R Bellare, H T Davis, W G Miller and L E Scriven,J. Colloid Interface Sci. 136, 305 (1990)
O D Lavrentovich and D-K Yang,ALCOM Symposium on reflective displays, Technical Reports IX, 11 (1997)
O D Lavrentovich, S V Shiyanovskii and D Voloschenko,Proceedings of SPIE — The International society for optical engineering 3787, 149 (1999)
D Voloschenko and O D Lavrentovich,Opt. Lett. 25, 317 (2000)
I I Smalyukh, S V Shiyanovskii and O D Lavrentovich,Chem. Phys. Lett. 336, 88 (2001)
S V Shiyanovskii, I I Smalyukh and O D Lavrentovich, in: Defects in liquid crystals: Computer simulations, theory and experiment edited by O D Lavrentovich, P Pasini, C Zannoni and S Zumer (Kluwer Academic Publishers, The Netherlands, 2001) vol. 43, pp. 229–270
D Voloschenko, O P Pishnyak, S V Shiyanovskii and O D Lavrentovich,Phys. Rev. E65, 060701 (2002)
I I Smalyukh and O D Lavrentovich,Phys. Rev. E66, 051703–16 (2002)
I I Smalyukh and O D Lavrentovich,Phys. Rev. Lett. 90, 085503 (2003)
T Wilson,Confocal microscopy (Academic Press, London, UK, 1990)
T R Corle and G S Kino,Confocal scanning optical microscopy and related imaging systems (Academic Press, San Diego, CA, 1996)
C J R Sheppard and D M Shotton,Confocal laser scanning microscopy (BIOS Scientific Publishers, Oxford, 1997)
R H Webb,Rep. Prog. Phys. 59, 427 (1996)
W R White and P Wiltzius,Phys. Rev. Lett. 75, 3012 (1995)
J Korlach, P Schwille, W W Webb and G W Feigenson,Proc. Natl. Acad. Sci. USA 96, 8461 (1999)
J B Nephew, T C Nihei and S A Carter,Phys. Rev. Lett. 80, 3276 (1998)
M H Chestnut,Current Opin. Colloid Interface Sci. 2, 158 (1997)
B V R Tata and B Raj,Bull. Mater. Sci. 21, 263 (1997)
G A Held, L L Kosbar, I Dierking, A C Lowe, G Grinstein, V Lee and R D Miller,Phys. Rev. Lett. 79, 3443 (1997)
K Amudson, A van Blaaderen and P Wiltzius,Phys. Rev. E55, 1646 (1997)
V J Anderson, E M Terentjev, S P Meeker, J Crain and W C K Poon,Euro. Phys. J. E4, 11 (2001)
M Srinivasarao, D Collings, A Philips and S Patel,Science 292, 79 (2001)
L M Blinov and V G Chigrinov,Electrooptic effects in liquid crystal materials (Springer, New York, 1993)
W E Ford and P V Kamat,J. Phys. Chem. 91, 6373 (1987)
M Kleman and O D Lavrentovich,Soft matter physics: An introduction (Springer, New York, 2003)
I Janossy,Phys. Rev. E49, 2957 (1994)
S V Shiyanovskii and Ju G Terentieva,Phys. Rev. E49, 916 (1994)
I I Smalyukh, R Pratibha, O D Lavrentovich and N V Madhusudana,Liq. Cryst. (2003) (submitted)
P A Pramod, R Pratibha and N V Madhusudana,Curr. Sci. 73, 761 (1997);Liq. Cryst. 28, 525 (2001)
T Tanaami, S Otsuki, N Tomosada, Y Kosugi, M Shimizu and H Ishida,Appl. Opt. 41, 4704 (2002)
P F McManamon, T A Dorschner, D L Corkum, L J Friedman, D S Hobbs, M Holz, S Lieberman, H Q Nguyen, D P Resler, R C Sharp and E A Watson,Proc. IEEE 84, 268 (1996)
D Subacius, S V Shiyanovskii, P Bos and O D Lavrentovich,Appl. Phys. Lett. 71, 3323 (1997)
I Dierking,Chem. Phys. Chem. 2, 663 (2001)
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Lavrentovich, O.D. Fluorescence confocal polarizing microscopy: Three-dimensional imaging of the director. Pramana - J Phys 61, 373–384 (2003). https://doi.org/10.1007/BF02708317
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DOI: https://doi.org/10.1007/BF02708317