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Expression of a ribosome inactivating protein (curcin 2) inJatropha curcas is induced by stress

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Abstract

The open reading frame (ORF) encoding curcin 2 was cloned from total genomic and cDNA ofJatropha curcas leaves, which were treated by drought, temperature stress and fungal infection, by polymerase chain reaction (PCR) and reverse transcriptase (RT)-PCR amplification. The ORF has 927 bp that encodes a precursor protein of 309 amino acid residues. There are high similarities with curcin and the conserved domain of ribosome inactivating proteins (RIPs). Antiserum to curcin recognized one band of 32 kDa on Western blot of the leaves treated by temperature stresses at 4°C and 50°C and by fungal infections ofPestalotia funerea, Curvularia lunata (Walk) Boed,Gibberelle zeae (Schw.) Petch. Two bands of 32 kDa and 65 kDa were recognized on Western blot of the leaves treated by 10%-40% polyethylene glycol (PEG). In addition, the 32 kDa band is nearly the molecular weight of curcin 2. This finding suggests that the protein of 32 kDa should be related to curcin 2. The presence of this protein molecular marker under stresses may provide an experimental foundation to study the stress proteins inJ. curcas.

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Abbreviations

ORF:

open reading frame

PEG:

polyethylene glycol

RIPs:

ribosome inactivating proteins

RT-PCR:

reverse transcriptase-polymerase chain reaction

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Authors and Affiliations

  1. College of Life Sciences, Sichuan University, 610 065, Chengdu, P R China

    Huang Ming-Xing, Xu Ying & Chen Fang

  2. Department of Biological Engineering, Yibin University, 644 007, Yinbin, P R China

    Wei Qin

  3. College of Light Industry and Food Engineering Sciences, Sichuan University, 610 065, Chengdu, P R China

    Wei Qin & Zhang Xin-Shen

Authors
  1. Wei Qin
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  2. Huang Ming-Xing
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  3. Xu Ying
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  4. Zhang Xin-Shen
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  5. Chen Fang
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Correspondence to Chen Fang.

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Qin, W., Ming-Xing, H., Ying, X. et al. Expression of a ribosome inactivating protein (curcin 2) inJatropha curcas is induced by stress. J. Biosci. 30, 351–357 (2005). https://doi.org/10.1007/BF02703672

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  • DOI: https://doi.org/10.1007/BF02703672

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