Abstract
A survey was conducted to study the biological and genetic diversity ofCardamom mosaic virus (CdMV) that causes the most widespread disease in the cardamom growing area in the Western Ghats of south India. Six distinct subgroups were derived based on their symptomatology and host range from the sixty isolates collected. The serological variability between the virus isolates was analysed by ELISA and Western blotting. The 3′ terminal region consisting of the coat protein (CP) coding sequence and 3′ untranslated region (3′UTR) was cloned and sequenced from seven isolates. Sequence comparisons revealed considerable genetic diversity among the isolates in their CP and 3′UTR, making CdMV one of the highly variable members ofPotyviridae. The possible occurrence of recombination between the isolates and the movement of the virus in the cardamom tract of south India are discussed.
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Abbreviations
- CdMV:
-
Cardamom mosaic virus
- CP:
-
coat protein
- PCR:
-
polymerase chain reaction
- 3′UTR:
-
3′ untranslated region
- YMV:
-
Yam mosaic virus
References
Aleman-Verdaguer M E, Goudou-Urbino C, Dubern J, Beachy R N and Fauquet C 1997 Analysis of sequence diversity of P1, HC, P3, NIb and CP genomic regions of several yam mosaic potyvirus isolates: implications for intraspecies molecular diversity of potyviruses;J. Gen. Virol. 78 1253–1264
Bateson M F, Henderson J, Chaleeprom W, Gibbs A J and Dale J L 1994 Papaya ringspot potyvirus, isolate variability and the origin of PRSV type P (Australia);J. Gen. Virol. 75 3547–3553
Bateson M F, Lines, R E, Revill P, Chaleeprom W, Ha C V, Gibbs A J and Dale J L 2002 On the evolution and molecular epidemiology of the potyvirusPapaya ringspot virus;J. Gen. virol. 83 2575–2585
Bousalem M, Douzery E J P and Fargette D 2000 High genetic diversity, distant phylogenetic relationships and intraspecies recombination events among natural populations of Yam mosaic virus, a contribution to understanding potyvirus evolution;J. Gen. Virol. 81 243–255
Carninci P, Nishiyama Y, Westover A, Itoh M, Nagaoka S, Sasaki N, Okazaki Y, Muramatsu M and Hayashizaki Y 1998 Thermo-stabilization and thermo-activation of thermo-labile enzymes by trehalose and its application for synthesis of full length cDNA;Proc. Natl. Acad. Sci. USA 95 520–524
Cervera M T, Riechmann J L, Martin M T and Garcia J A 1993 3’-Terminal sequence of the plum pox virus PS and o6 isolates: evidence for RNA recombination within the potyvirus group;J. Gen. Virol. 74 329–334
Desbiez C, Wipf-Scheibel and Lecoq H 2002 Biological and serological variability, evolution and molecular variability of Zucchini yellow mosaic virus (ZYMV, potyvirus) with special reference to Caribbean islands;Virus Res. (in press)
Farrell R E Jr 1993 RNAMethodologies: A laboratory guide for isolation and characterization (San Diego, California: Academic Press)
Felsenstein J 1989 PHYLIP-Phylogeny Inference Package (version 3.2);Cladistics 5 164 (http://evolution.genetics.washington.edu/phylip.html)
Frenkel M J, Ward C W and Shukla D D 1989 The use of 3′non coding nucleotide sequences in the taxonomy of potyviruses: application to watermelon mosaic virus 2 and Soybean mosaic virus-N;J. Gen. Virol. 70 2775–2783
Frenkel M J, Jilka J M, Shukla D D and Ward C W 1992 Differentiation of potyviruses and their strains by hybridization with the 3′ non-coding region of the viral genome;J. Virol. Methods 36 51–62
Gal-On A, Meiri E, Raccah B and Gaba V 1998 Recombination of engineered defective RNA species produces infective potyvirusin planta;J. Virol. 72 5268–5270
Jacob T and Usha R 2001 3′ terminal sequence analysis of the RNA genome of the Indian isolate ofCardamom mosaic virus: A new member of the genusMacluravirus ofPotyviridae;Virus Genes 23 81–88
Jacob T and Usha R 2002 Expression ofCardamom mosaic virus coat protein inEscherichia coli and its Assembly into filamentous aggregates;Virus Res. 86 133–141
Gibbs A and Mackenzie A 1997 A primer pair for amplifying part of the genome of all potyvirids by RT-PCR;J. Virol. Methods 63 9–16
Mayne M W 1951 A report on cardamom cultivation in South India;Bulletin (ICAR, New Delhi)50 p 62
Shukla D D, Ward C W and Brunt A A 1994The Potyviridae (Wallingford: CAB International)
Shukla D D and Ward C W 1988 Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group;J. Gen. Virol. 69 2703–2710
Thompson J D, Higgins D G and Gibson T J 1994 CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice;Nucleic Acids Res. 22 4673–4680
Uppal B N, Varma P M and Capoor S P 1945 Mosaic disease of cardamom;Curr. Sci. 14 208–209
Varma P M 1962 The banana aphid (Pentalonia nigronervosa Coq.) and the transmission of ‘katte’ disease of cardamom;Indian Phytopathol. 15 1–10
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Jacob, T., Jebasingh, T., Venugopal, M.N. et al. High genetic diversity in the coat protein and 3′ untranslated regions among geographical isolates ofCardamom mosaic virus from south India. J. Biosci. 28, 589–595 (2003). https://doi.org/10.1007/BF02703334
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DOI: https://doi.org/10.1007/BF02703334