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[125I] gonadotropin binding to the ovary of an Indian major carp,Catla catla, at different stages of reproductive cycle

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Abstract

At different stages of the annual reproductive cycle ofCatla catla, a major Indian carp, specific binding of gonadotropic hormone to the plasma membrane receptors was demonstrated. Maximum specific binding of [125I]Catla gonadotropic hormone was obtained at 30‡C and pH 7.5 during 2 h of incubation.Catla gonadotropic hormone binding was saturable with high affinity. Competitive inhibition experiment showed that binding site was specifically occupied by piscine gonadotropic hormone,Catla gonadotropic hormone and murrel gonadotropic hormone, human chorionic gonadotropin was a weak competitor while bovine thyroid stimulating hormone, bovine prolactin and ovine follicle stimulating hormone had no effect. Scatchard analysis ofCatla gonadotropic hormone binding to the plasma membrane preparation from the carp oocytes of different reproductive stages showed that the range of dissociation constant(Kd) varied from 0.78 to 0.97 x 10-10 M. However, maximum binding capacity (B-max) varied remarkably between the different stages of reproductive cycle, it was 6.11 ± 0.36 fmol/mg protein in the preparatory stage which increased to about three-fold in prespawning stage of reproductive cycle (17.0 ± 0.29 fmol/mg protein) and spawning (18.7 ± 0.17 fmol/mg protein) and lowest in postspawning stage of reproductive cycle (5.28 ± 0.28 fmol/mg protein). Fluctuation in the number of gonadotropic hormone binding site at different stages of annual reproductive cycle was found to be coincided well with the pattern of ovarian steroidogenesis in response toCatla gonadotropic hormone as determined by the formation of progesterone from pregnenolone.

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Abbreviations

GTH:

Gonadotropic hormone

cGTH:

Catla gonadotropic hormone

hCG:

human chorionic gonadotropin

bTSH:

bovine thyroid stimulating hormone

bPRL:

bovine prolactin

oFSH:

ovine follicle stimulating hormone

PEG:

polyethylene glycol

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  1. Department of Zoology, School of Life Sciences, Visva-Bharati University, 731 235, Santiniketan, India

    Pulak Ranjan Manna & Samir Bhattacharya

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  1. Pulak Ranjan Manna
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  2. Samir Bhattacharya
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Manna, P.R., Bhattacharya, S. [125I] gonadotropin binding to the ovary of an Indian major carp,Catla catla, at different stages of reproductive cycle. J. Biosci. 18, 361–372 (1993). https://doi.org/10.1007/BF02702994

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